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Carolina BioKits®: Introduction to Gene Regulation: The lac Operon (with perishables)

Item # 171027P Exclusive

Introduce your students to gene regulation with this lab activity focused on the lac operon. During the lab, students test the B-galactosidase levels of 3 cultures grown in the presence of glucose, lactose, or glucose and lactose. Perform as a quantitative (with spectrophotometer) or qualitative lab. Designed for 8 groups of 2 to 4 students.

Price

$96.25

In stock and available to ship.

Introduce your students to gene regulation with this lab activity focused on the lac operon. During the lab, students test the B-galactosidase levels of 3 cultures grown in the presence of glucose, lactose, or glucose and lactose. You have the option of allowing students to perform the activity as a quantitative lab (with spectrophotometer) or as a qualitative lab (without spectrophotometer). This kit is designed for students working in 8 groups of 2 to 4. Note: Kit includes perishable materials. The o-nitrophenyl-B-D-galactopyranoside must be kept frozen.

  • This item is only available from Carolina Biological Supply Company.
  • This item contains living or perishable material and ships via 2nd Day or Overnight delivery to arrive on a date you specify during Checkout. To ensure freshness during shipping, a Living Materials Fee may apply to orders containing these items.
  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.
 

This item ships with living or perishable materials (all items). Please select a delivery date during Checkout.

Components Qty Included?
Escherichia coli Slant Culture   1 Included
ONPG, 0.3 g   1 Included
Incomplete Medium, 60 mL 3 Included
Lactose, 0.3 g 1 Included
Glucose, 0.3 g 1 Included
Mixture of Lactose and Glucose 1 Included
Inoculating Loop 1 Included
Disposable Pipets 40 Included
Blank Labels 100 Included
Teacher's Manual and Reproducible Student Guide 1 Included
Test Tube Covers (e.g., caps, stoppers, Parafilm®) 24 Needed, Not included
Containers (for ONPG, optional) 3 Needed, Not included
Permanent Markers 8 Needed, Not included
Bleach Needed, Not included
Ethanol or Other Disinfectant Needed, Not included
Incubator, 37°C 1 Needed, Not included
Bunsen Burner 1-8 Needed, Not included
Test Tubes (qualitative protocol) 24 Needed, Not included
Test Tube Racks 8 Needed, Not included
Test Tubes (quantitative protocol) 48 Needed, Not included
Spectrophotometer for measuring A600, A420, and A550 (quantitative protocol) 1 Needed, Not included
Cuvettes (quantitative protocol) Needed, Not included
Serological Pipets, for measuring 2.0 mL, 2.4 mL, 4.5 mL (quantitative protocol) Needed, Not included
Pipetting Device for Serological Pipets (quantitative protocol) Needed, Not included

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Correlations to State Standards

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