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Introduce your students to gene regulation with this lab activity focused on the lac operon. During the lab, students test the B-galactosidase levels of 3 cultures grown in the presence of glucose, lactose, or glucose and lactose. Perform as a quantitative (with spectrophotometer) or qualitative lab. Designed for 8 groups of 2 to 4 students.
Introduce your students to gene regulation with this lab activity focused on the lac operon. During the lab, students test the B-galactosidase levels of 3 cultures grown in the presence of glucose, lactose, or glucose and lactose. You have the option of allowing students to perform the activity as a quantitative lab (with spectrophotometer) or as a qualitative lab (without spectrophotometer). This kit is designed for students working in 8 groups of 2 to 4. Note: Kit includes perishable materials. The o-nitrophenyl-B-D-galactopyranoside must be kept frozen.
|Escherichia coli Slant Culture||1||Included|
|ONPG, 0.3 g||1||Included|
|Incomplete Medium, 60 mL||3||Included|
|Lactose, 0.3 g||1||Included|
|Glucose, 0.3 g||1||Included|
|Mixture of Lactose and Glucose||1||Included|
|Teacher's Manual and Reproducible Student Guide||1||Included|
|Test Tube Covers (e.g., caps, stoppers, Parafilm®)||24||Needed, Not included|
|Containers (for ONPG, optional)||3||Needed, Not included|
|Permanent Markers||8||Needed, Not included|
|Bleach||Needed, Not included|
|Ethanol or Other Disinfectant||Needed, Not included|
|Incubator, 37°C||1||Needed, Not included|
|Bunsen Burner||1-8||Needed, Not included|
|Test Tubes (qualitative protocol)||24||Needed, Not included|
|Test Tube Racks||8||Needed, Not included|
|Test Tubes (quantitative protocol)||48||Needed, Not included|
|Spectrophotometer for measuring A600, A420, and A550 (quantitative protocol)||1||Needed, Not included|
|Cuvettes (quantitative protocol)||Needed, Not included|
|Serological Pipets, for measuring 2.0 mL, 2.4 mL, 4.5 mL (quantitative protocol)||Needed, Not included|
|Pipetting Device for Serological Pipets (quantitative protocol)||Needed, Not included|
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