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Introduce your students to gene regulation with this lab activity focused on the lac operon. During the lab, students test the B-galactosidase levels of 3 cultures grown in the presence of glucose, lactose, or glucose and lactose. Perform as a quantitative or qualitative lab. Designed for 8 groups of 2 to 4 students. Note: Kit includes a prepaid coupon for perishables. Contact us or return coupon to request delivery of perishables.
Introduce your students to gene regulation with this lab activity focused on the lac operon. During the lab, students test the B-galactosidase levels of 3 cultures grown in the presence of glucose, lactose, or glucose and lactose. You have the option of allowing students to perform the activity as a quantitative lab (with spectrophotometer) or as a qualitative lab (without spectrophotometer). This kit is designed for students working in 8 groups of 2 to 4. Note: Kit includes a prepaid coupon to request the perishables later at your convenience. Contact us or return the coupon to request delivery of perishable materials. The o-nitrophenyl-B-D-galactopyranoside must be kept frozen.
|Escherichia coli Slant Culture||1||Included|
|ONPG, 0.3 g||1||Included|
|Incomplete Medium, 60 mL||3||Included|
|Lactose, 0.3 g||1||Included|
|Glucose, 0.3 g||1||Included|
|Mixture of Lactose and Glucose||1||Included|
|Teacher's Manual and Reproducible Student Guide||1||Included|
|Test Tube Covers (e.g., caps, stoppers, Parafilm®)||24||Needed, Not included|
|Containers (for ONPG, optional)||3||Needed, Not included|
|Permanent Markers||8||Needed, Not included|
|Bleach||Needed, Not included|
|Ethanol or Other Disinfectant||Needed, Not included|
|Incubator, 37°C||1||Needed, Not included|
|Bunsen Burner||1-8||Needed, Not included|
|Test Tubes (qualitative protocol)||24||Needed, Not included|
|Test Tube Racks||8||Needed, Not included|
|Test Tubes (quantitative protocol)||48||Needed, Not included|
|Spectrophotometer for measuring A600, A420, and A550 (quantitative protocol)||1||Needed, Not included|
|Cuvettes (quantitative protocol)||Needed, Not included|
|Serological Pipets, for measuring 2.0 mL, 2.4 mL, 4.5 mL (quantitative protocol)||Needed, Not included|
|Pipetting Device for Serological Pipets (quantitative protocol)||Needed, Not included|
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