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This kit introduces students to the use of biochemical characteristics for differentiating 4 closely related bacteria. Citrate utilization, hydrogen sulfide production, butanediol fermentation, and mixed acid fermentation tests are conducted to identify an unknown bacteria. Tests are completed in 5 days. Keep bacteria cultures, reagents, and agar at room temperature. Perishable cultures included. Enough materials for a class of 30.
|Citrobacter freundii agar slant culture||Included|
|Enterobacter aerogenes agar slant culture||Included|
|Escherichia coli agar slant culture||Included|
|Serratia liquefaciens agar slant culture||Included|
|Unknown bacteria agar slant culture (E. coli)||Included|
|Tubes Kligler's iron agar||5||Included|
|Tubes Simmons citrate agar||5||Included|
|Tubes MR-VP broth||5||Included|
|Methyl Red Reagent||Included|
|Barrit Reagent A||Included|
|Barrit Reagent B||Included|
|Sterile Test Tubes||5||Included|
|Sterile Serological Pipets (1 mL)||5||Included|
|Dropping Pipets (1 mL)||15||Included|
|Metal Inoculating Loop||Included|
|Metal Inoculating Needle||Included|
|Autoclave Disposal Bag||Included|
|37 ° C Incubator||Needed, Not included|
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