Genetic Construction Kit
A safe classroom exercise in which students produce a new strain of viable bacteria. The genetic manipulation conducted is similar to more complex procedures common in modern genetic engineering. Through bacterial conjugation, substituted sex factors (F plasmids bearing lac operon genes) are transferred to a background genome expressing antibiotic resistance. Indicator and selective agar media are used to identify and isolate the hybrid cells. Cultures require incubation. Designed for 6 teams.