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DNA Barcode Amplification Kit (with Prepaid Coupon)

Item # 211385 Exclusive

Price

$204.95

In stock and available to ship.

  • Use DNA barcodes to identify student-collected samples of plants or animals
  • Enjoy robust and simple protocol designed with the classroom in mind
  • Connect students to real-world bioinformatics
  • Identify diversity by taking taxonomy to the next level
This kit explores an emerging trend in molecular biology and brings bioinformatics to the classroom in an accessible manner. Students take a new approach to taxonomy using "DNA barcodes"—short, unique DNA sequences—to learn about the biodiversity of plants, mammals, fish, or insects. Using polymerase chain reaction (PCR) on specific regions of the rbcL or COI gene, students amplify their extracted DNA to create unique barcodes. These amplified sequences (amplicons) are then submitted for sequencing and can be analyzed using online tools. Our partner, GENEWIZ, Inc., offers an educational discount for sequencing services. Each kit contains materials for 25 DNA extractions and PCR reactions.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

  • This item is only available from Carolina Biological Supply Company.
  • Excepted Quantity - This product is/contains a DOT regulated hazardous material in an excepted quantity. This does not incur a hazardous materials fee. Orders shipping to HI and AK are subject to review and may incur additional shipping charges.
  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses.

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This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
Plant rcbL Primer/Loading Dye Mix, 700 μL 1 Included
Fish COI Primer/Loading Dye Mix, 700 μL 1 Included
Insect-mammal Primer/Loading Dye Mix, 700 μL 1 Included
RNAse Solution, 250 μL 1 Included
pBR322/BstNI Markers, 0.075 μg/μL, 130 μL 1 Included
Nuclei Lysis Solution, 20 mL 1 Included
Protein Precipitation Solution, 7 mL 1 Included
Isopropanol, 100%, 20 mL 1 Included
Ethanol, 70%, 30 mL 1 Included
DNA Rehydration Buffer, 3.5 mL 1 Included
Mineral Oil, 5 mL 1 Included
Ready-to-Go™ PCR Beads (0.2 mL tubes) 25 Included
Plastic Pestles 25 Included
PCR 8-tube Strips 4 Included
Teacher's Manual and Reproducible Student Guide 1 Included
Collection Tubes, Jars, or Bags Needed, Not included
Tweezers/Scissors/Scalpel Needed, Not included
Water Baths or Heatblocks, 37°C and 65°C Needed, Not included
Micropipets and Tips, 1 μL to 1000 μL Needed, Not included
Microcentrifuge for 1.5 mL Tubes Needed, Not included
Microcentrifuge Tubes, 1.5 mL Needed, Not included
Microcentrifuge Tube Racks Needed, Not included
Programmable Thermal Cycler Needed, Not included
Electrophoresis Chambers Needed, Not included
Electrophoresis Power Supplies Needed, Not included
UV Transilluminator or White Light Box Needed, Not included
Permanent Markers Needed, Not included
Container with Cracked or Crushed Ice Needed, Not included
Computer with Internet Access Needed, Not included
Vortexer (optional) Needed, Not included
Camera or Photo-documentary System (optional) Needed, Not included
Smarthphone or Camera with GPS (optional) Needed, Not included

Correlations to State Standards

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    Carolina Biological Supply

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    2700 York Road, Burlington, NC 27215-3398 • 800.334.5551