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DNA Barcode Amplification Kit (with prepaid coupon)

Item # 211385 Exclusive

Advanced—For experienced high school and college classes; requires some technical skill.
Conduct cutting-edge science in your classroom, simply and easily. The DNA Barcode Amplification Kit is an unmatched opportunity to give students hands-on exposure to PhD-level science research. Its simple procedures and extensive directions have been updated, based on teacher feedback, to save you time and clarify the protocol. Kit is designed for 25 PCR reactions.

Price

$204.95

In stock and available to ship.

Advanced—For experienced high school and college classes; requires some technical skill.

  • Revised protocol saves you time and delivers robust results
  • Use DNA barcodes to identify student-collected samples of plants or animals
  • Designed, tested, and updated with the classroom in mind
  • Connect students to real-world bioinformatics
  • Identify diversity by taking taxonomy to the next level
This kit explores an emerging trend in molecular biology and brings bioinformatics to the classroom in an accessible manner. Students take a new approach to taxonomy using "DNA barcodes"—short, unique DNA sequences—to learn about the biodiversity of plants, mammals, fish, or insects. Unlock the barcodes of even more organisms by purchasing the Fungal Primer Set (item #211388, sold separately) to give students the opportunity to explore the diverse world of fungi. Using polymerase chain reaction (PCR) on specific regions of the rbcL or COI gene, students amplify their extracted DNA to create unique barcodes. These amplified sequences (amplicons) are then submitted for sequencing and can be analyzed using our custom-designed online tool, DNA Subway. Our partner, GENEWIZ, Inc., offers an educational discount for sequencing services. Kit contains materials for 25 DNA extractions and PCR reactions. Electrophoresis reagents need to be purchased separately.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses.

  • This item is only available from Carolina Biological Supply Company.
  • Excepted Quantity - This product is/contains a DOT regulated hazardous material in an excepted quantity. This does not incur a hazardous materials fee. Orders shipping to HI and AK are subject to review and may incur additional shipping charges.
  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses.

Product Families Featuring This Item:

 

This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
Plant rcbL Primer/Loading Dye Mix, 700 μL 1 Included
Fish COI Primer/Loading Dye Mix, 700 μL 1 Included
Insect-Mammal Primer/Loading Dye Mix, 700 μL 1 Included
pBR322/BstNI Markers, 0.075 μg/μL, 130 μL 1 Included
Silica Resin, 200 μL 1 Included
Nuclei Lysis Solution, 10 mL 1 Included
Wash Buffer Concentrate, 23.7 mL 1 Included
Sterile Distilled Water, 4 mL 1 Included
Ethanol, 95%, 30 mL 1 Included
Mineral Oil, 5 mL 1 Included
Ready-to-Go™ PCR Beads (0.2-mL tubes) 25 Included
Plastic Pestles 25 Included
PCR 8-Tube Strips 4 Included
Teacher's Manual with Reproducible Student Guide 1 Included
Collection Tubes, Jars, or Bags Needed, Not Included
Tweezers/Scissors/Scalpel Needed, Not Included
Water Baths or Heat Blocks, 37° C and 65° C Needed, Not Included
Micropipets and Tips, 1 to 1,000 μL Needed, Not Included
Microcentrifuge for 1.5-mL Tubes Needed, Not Included
Microcentrifuge Tubes, 1.5 mL Needed, Not Included
Microcentrifuge Tube Racks Needed, Not Included
Programmable Thermal Cycler Needed, Not Included
Electrophoresis Chambers Needed, Not Included
Electrophoresis Power Supplies Needed, Not Included
Electrophoresis Reagents Needed, Not Included
UV Transilluminator or White Light Box Needed, Not Included
Permanent Markers Needed, Not Included
Container with Cracked or Crushed Ice Needed, Not Included
Computer with Internet Access Needed, Not Included
Safety Goggles Needed, Not Included
Vortexer (optional) Needed, Not Included
Camera or Photo-Documentary System (optional) Needed, Not Included
Smartphone or Camera with GPS (optional) Needed, Not Included

Correlations to State Standards

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    DNA Learning Center

    Cutting Edge Science in your Classroom

    Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.

    Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.

    At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.


    About the DNA Learning Center

    The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.

    Carolina Biological Supply

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