By Dr. Greg Freyer (coauthor of DNA Science: A First Course2nd Edition, item# 212209) exclusively for Carolina. Plasmid contains gene for ampicillin resistance and the entire ß-galactosidase gene. It doesn't depend on complementing a partial ß-galactosidase gene product as does pUC. The host, E. coli DH5, doesn't need to be maintained on minimal medium for pBLU® transformation. The lac suppressor gene is inactivated, eliminating need for IPTG in the medium.
pBLU® Plasmid, 10 µg (50 µL; 0.2 µg/µL)
pBLU® Plasmid, 20 µg (100 µL; 0.2 µg/µL)
pBLU® Plasmid, 20 µg (50 µL; 0.4 µg/µL)
pBLU® Plasmid, 1 µg (200 µL; 0.005 µg/µL)