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Students use a standard curve to analyze data in this experiment. They gather data by performing a BCA assay to determine the amount of protein in a standard versus 3 different food samples (included). Optionally, they can supply and test a fourth liquid food sample of their choosing.
Intermediate - Easy to perform; requires some background knowledge.
Students use a standard curve to analyze data in this experiment. They gather data by performing a BCA assay to determine the amount of protein in a standard versus 3 different food samples (included). Optionally, they can supply and test a fourth liquid food sample of their choosing. A spectrophotometer is used to obtain numerical values and absorbances of dilutions of the standard and the samples. Students graph absorbance versus protein concentration for the standard and use the resulting curve to interpolate the concentration of protein in the samples. Kit includes enough materials for 8 stations of students.
|Reagent A, 305 mL||1||Included|
|Reagent B, 7 mL||1||Included|
|BSA Standard, 2 mg/mL, 1.4 mL||1||Included|
|High-protein Health-food Drink||1||Included|
|BSA Dilution Tubes (1.5 mL microcentrifuge tubes)||48||Included|
|Assay and Food Product Dilution Tubes, 15 mL||136||Included|
|Pipets, 5 mL||8||Included|
|Pipets, 10 mL||8||Included|
|Teacher's Manual and Reproducible Student Guide||1||Included|
|Containers for Holding 37 mL of Reagent A||8||Needed, Not Included|
|Containers for Holding 0.780 mL of Reagent B||8||Needed, Not Included|
|Rulers||8||Needed, Not Included|
|Trays||8||Needed, Not Included|
|Spectrophotometer(s) (absorbance at 562 nm)||Needed, Not Included|
|Pipet Bulb or other Pipetting Device||Needed, Not Included|
|Pipets to measure 20 to 780 μL||Needed, Not Included|
|Water Baths, 37 or 60°C||Needed, Not Included|
|Distilled or Deionized Water||Needed, Not Included|
|Racks||Needed, Not Included|
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