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Inducing RNAi by Feeding Kit

3 Items
$4.95 - $175.00 View Details

Advanced – For experienced high school and college classes; requires some technical skill.
Demonstrate to your students the power of silencing a single gene. RNA interference (RNAi) is a technique that allows you to silence the expression of a chosen gene by specifically degrading its mRNA. In this kit, students use this Nobel Prize-winning technique to silence 2 different genes in the nonparasitic roundworm C. elegans, a model organism used for studying many aspects of biology.

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Advanced – For experienced high school and college classes; requires some technical skill.

  • Demonstrate to your students the power of silencing a single gene.
  • Teach about a powerful method for determining gene function.
  • Introduce your students to a model organism used for studying many aspects of biology, including development and gene function.
  • Engage in bioinformatics exercises exploring protein function and C. elegans and human gene relatedness.
In this lab, wild-type worms are fed lab strains of E. coli that express double-stranded RNA (dsRNA) corresponding to either of 2 target genes: BLIstered cuticle (bli-1) or DumPY (dpy-11). The ingestion of the dsRNA initiates the destruction of mRNA expressed from the target genes. Students then examine the phenotypes induced by silencing these genes. Silencing bli-1 leads to the formation of clear blisters in a worm’s outer cuticle; silencing dpy-11 leads to a worm developing a short, fat body. In accompanying bioinformatics exercises, students explore the function of the protein encoded by the silenced gene and the relatedness of worm and human genes.

Note: Order the kit with the perishable materials included (item #211391P) or with a prepaid coupon to request perishables later at your convenience (item #211391). Because of the time-sensitive preparation and limited shelf life of the kit’s perishable materials, please order a minimum of 2 weeks prior to the desired ship date. Plan on using the materials within a week of receiving them. Perishables ship only on Mondays, Tuesdays, and Wednesdays via standard overnight delivery.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

 
 
 
 
 
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