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Examining the RNAi Mechanism Kit (RNAi and Amplification; with Prepaid Coupon)

Item # 211392

RNA interference (RNAi) is a technique that allows you to silence the expression of a chosen gene by specifically degrading the gene's mRNA. Students use the Nobel Prize-winning technique to silence the dpy-13 gene in the nonparasitic roundworm C. elegans.

Price

$315.00

In stock and available to ship.

Advanced - For experienced high school and college classes; requires some basic technical skill.

  • Demonstrate to your students the power of silencing a single gene
  • Teach about a powerful method for determining gene function
  • Explore the relationship between phenotype, genotype, and RNAi-induced phenotype
  • Perform bioinformatics exercises exploring protein function and C. elegans, and human relatedness
RNA interference (RNAi) is a technique that allows you to silence the expression of a chosen gene by specifically degrading the gene's mRNA. Examining the RNAi Mechanism kit allows students to use this Nobel Prize-winning technique to silence the dpy-13 gene in the nonparasitic roundworm C. elegans. They observe wild-type worms eat a lab strain of E. coli that expresses double-stranded RNA (dsRNA) corresponding to the targeted gene, dumpy 13 (dpy-13).

Kit includes a prepaid coupon to request perishable materials later at your convenience. Contact us or return the coupon to request delivery of perishables.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.

Product Families Featuring This Item:

 

This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
Ampicillin, 10 mg/mL, 4 mL 1 Included
Cholesterol, 5 mg/mL, 0.3 mL 2 Included
IPTG, 0.036 g 1 Included
Proteinase K, 20 mg/mL, 25 μL 1 Included
DNA Marker pBR322/BstN1, 200 μL 1 Included
dpy-13 (e458) Worms on NGM-lite Plates 4 Included
dpy-13 Primer/Loading Dye Mix, 700 μL 1 Included
E. coli RNA Feeding Strain (dpy-13) LB Amp/Tet Slant 1 Included
E. coli OP50 on LB Slant 1 Included
Wild-type Worms on NGM-lite Plates 4 Included
1x Lysis Buffer, 0.5 mL 1 Included
Sterile Bulb Pipets 6 Included
Sterile Culture Tubes, 15 mL 3 Included
Sterile Inoculating Loops 3 Included
LB Broth, 3 mL 3 Included
Mineral Oil, 5 mL 1 Included
Melt 'n Pour NGM-lite, 300 mL 1 Included
Melt 'n Pour NGM-lite, 100 mL 1 Included
Sterile Petri Dishes, 60 mm 40 Included
Ready-to-Go™ PCR Beads (0.2 mL tubes) 25 Included
Sterile Distilled Water, 1 mL 1 Included
Sterile Toothpicks 8 Included
Teacher's Manual and Reproducible Student Guide 1 Included
Stereomicroscope Needed, Not included
Bunsen Burner Needed, Not included
Containers with Cracked or Crushed Ice Needed, Not included
Container with Liquid Nitrogen or Dry Ice Needed, Not included
Digital or Instant Camera (optional) Needed, Not included
Ethanol, 95% Needed, Not included
Gel Casting Trays and Combs Needed, Not included
Gel Electrophoresis Chamber Needed, Not included
Masking Tape Needed, Not included
Microcentrifuge Needed, Not included
Microcentrifuge Adaptors Needed, Not included
Micropipettor and Tips, 1-20 μL and 20-200 μL Needed, Not included
Microcentrifuge Tube Rack Needed, Not included
Mineral Oil Needed, Not included
PCR Tubes, 0.2 or 0.5 μL Needed, Not included
Permanent Markers, Black and Red Needed, Not included
Power Supply Needed, Not included
Steel Scalpel, Spatula, or Forceps Needed, Not included
Thermal Cycler Needed, Not included
UV or White Light Transilluminator Needed, Not included
Water Bath, 60°C (for agarose solution) Needed, Not included
Heavy-duty Plastic Bag Needed, Not included
Household Bleach Needed, Not included

DNA Learning Center

Cutting Edge Science in your Classroom

Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.

Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.

At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.


About the DNA Learning Center

The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.

Correlations to State Standards

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