This vocabulary assessment is used as a pretest to determine your initial knowledge of biotechnology and as a posttest to determine your end-of-course knowledge. Write brief answers that define and explain the following terms. GOOD LUCK!
1.
3' end
2.
37° C
3.
42° C
4.
5' end
5.
95° C - 58° C - 72° C
6.
adenine
7.
adhesion zones
8.
agar
9.
agarose
10.
alleles
11.
amino acid
12.
ampicillin
13.
amplification
14.
antibiotic
15.
arrayed library
16.
aseptic technique
17.
ATP
18.
autoradiography
19.
autosome
20.
bacteriophage
21.
balance
22.
BamHI
23.
bands
24.
base pair
25.
base sequence
26.
base sequence analysis
27.
bioinformatics
28.
biotechnology
29.
bp
30.
buffer
31.
CaCl2
32.
Carolina BLU™
33.
casting tray
34.
cDNA
35.
centimorgan
36.
centromere
37.
chromosome
38.
clone bank
39.
clones
40.
cloning
41.
cloning vector
42.
cM
43.
codon
44.
competent cells
45.
complementary base
46.
conjugation
47.
conserved sequences
48.
contig map
49.
contigs
50.
control
51.
cosmid
52.
crossing over
53.
culture tube
54.
cytosine
55.
denaturation
56.
diploid
57.
DNA
58.
DNA extraction
59.
DNA replication
60.
DNA sequence
61.
DNA spooling
62.
dNTPs
63.
domain
64.
double helix
65.
E. coli
66.
EcoRI
67.
electrophoresis
68.
electrophoresis chamber
69.
endonuclease
70.
enzyme
71.
ethidium bromide
72.
eukaryote
73.
exogenous DNA
74.
exon
75.
exonuclease
76.
experimental factor
77.
expression
78.
forensic DNA
79.
gamete
80.
gel
81.
gene
82.
gene expression
83.
gene families
84.
gene library
85.
gene mapping
86.
gene mining
87.
gene product
88.
gene splicing
89.
genetic code
90.
genetics
91.
genome
92.
guanine
93.
haploid
94.
heterozygosity
95.
HindIII
96.
homeobox
97.
homologies
98.
homologous chromosomes
99.
human gene therapy
100.
Human Genome Project
101.
hybridization
102.
hydrogen bonds
103.
in situ hybridization
104.
in vitro
105.
incubation
106.
inoculating loop
107.
interface
108.
interphase
109.
intron
110.
jumping gene
111.
karyotype
112.
kb
113.
lambda
114.
lanes
115.
LB
116.
ligase
117.
linkage
118.
linkage map
119.
loading dye
120.
locus
121.
marker
122.
Mb
123.
meiosis
124.
metaphase
125.
microcentrifuge
126.
microliter
127.
micropipettor
128.
mitosis
129.
MM 294
130.
mRNA
131.
mtDNA
132.
mutation
133.
nick
134.
nitrogenous base
135.
nucleic acid
136.
nucleotide
137.
oncogene
138.
operon
139.
ori
140.
palindrome
141.
pAMP
142.
Parafilm®
143.
PCR
144.
pGREEN
145.
phage
146.
photodocumentation
147.
physical map
148.
PKU
149.
plasmid
150.
polyacrylamide gel
151.
polyadenation
152.
polygenetic disorder
153.
polymerase
154.
polymorphism
155.
power supply
156.
primer
157.
probe
158.
prokaryote
159.
promoter
160.
protein
161.
purine
162.
pyrimidine
163.
rDNA
164.
regulatory sequence
165.
restriction enzyme
166.
RFLP
167.
ribosome
168.
RNA
169.
rRNA
170.
sequencing
171.
sex chromosome
172.
somatic cells
173.
Southern blotting
174.
staining
175.
staining tray
176.
STR
177.
tandem repeat
178.
taq polymerase
179.
TBE
180.
TE
181.
telomere
182.
thermocycler
183.
thymine
184.
transcription
185.
transfer pipet
186.
transformation
187.
transformation tube
188.
transillumination system
189.
translation
190.
tRNA
191.
ultraviolet light
192.
uptake
193.
uracil
194.
vector
195.
virus
196.
VNTR
197.
water bath
198.
wells
199.
YAC
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