Stressed
about an upcoming science fair? You are not alone. Carolina
product managers often answer questions about potential projects
from anxious parents, students, and teachers. They usually want
suggestions for small, easy-to-maintain organisms that a student
would find interesting and like to use in an experiment. We can
help you get started.
Aside from consultations on organisms, we also offer advice on
the proper care and maintenance of the specimens, measures that
are often overlooked. It is important to allot adequate time for
research into the care of an organism, selection and acquisition
of the specimen, creation of its habitat, and daily maintenance
needs. The list below contains information on some of our most
popular organisms for science fair projects.
Known for their jerking movement through water,
Daphnia are ideal specimens for physiological exercises and
toxicity tests. The organisms are filter feeders that exhibit
behavior in the laboratory setting very similar to their natural
behavior. Though they are known as “water fleas,”
Daphnia are
not related to the terrestrial flea.
These organisms require special care. It is best to use
Daphnia cultures in experiments within 24 hours of receiving them. We
suggest introducing the organisms to the new environment by
gently submerging the opened shipping jar in their habitat, not
dumping the organisms from the shipping container. Submerging
the jar prevents air bubbles from collecting under the
organisms’ shells, which would lift them to the surface and
decrease their chances of survival.
Daphnia are sensitive to the chemistry of the water in their
habitat, so make sure the water source is either springwater or
filtered pond water. We strongly advise against the use of tap
water as it may contain chlorine levels that are lethal. Also
avoid distilled water, which does not have the essential
minerals required for osmotic equilibrium.
Environment plays a critical role in development. The organisms
are most successful when raised at room temperature and kept out
of direct sunlight.
Daphnia reproduce asexually and sexually,
but under ideal environmental conditions most populations will
consist of only females. When conditions become too stressful
(e.g., food shortage, poor water quality, extremes in
temperature, and overcrowding), males will be produced and
sexual reproduction will begin to take place.
Among the simplest types of multicellular organisms,
planarians are an excellent choice for regeneration studies.
The black and brown varieties (
Dugesia dactyligeria and
Dugesia tigrina) are the best options because they do not
take as long as the white planarians (
Dugesia morgani) to
regenerate.
The flatworms can be kept in an 8” culture dish or a
shallow, soap-free container that holds about ¾ to 2” of
springwater. Transfer the specimens to a fresh dish of
springwater each day. We do not recommend tap water because
it contains metal ions and chlorine, which are deadly to
planarians.
These self-healing organisms are highly sensitive to intense
light, temperature, and pH; therefore, it is important to
not make any drastic changes to these environmental factors
once you begin experimentation. When you receive your
planarians, do not feed them for the first week. After that
period, feed them foods such as fresh beef liver,
hard-boiled egg yolk,
Lumbriculus, and pieces of earthworm.
Lumbriculus variegatus, a common freshwater annelid, is
an excellent alternative to a planarian for regeneration
studies and water quality projects. The blackworms are
hardy and easy to raise either at home or in the
classroom.
Create a home for your worms by filling a pan or
aquarium with 2 to 3” of springwater (or aged and
dechlorinated tap water) at room temperature and lining
it with brown paper towels. Sinking fish food is the
worms’ food of choice, but they can survive bouts of
irregular feeding or long periods of starvation. In the
laboratory, populations can double in about 3 to 4
weeks.
Students with an understanding of genetics and an eye
for detail will find that
Drosophila melanogaster, the
common fruit fly, offers challenging and interesting
project ideas. Subjects can be as simple as the fruit
fly life cycle or as complex as sex-linked inheritance
of traits.
The laboratory-raised cultures are easy to use and
require few supplies for handling and rearing. Results
can be obtained in a reasonable amount of time. Within a
week of receiving your culture, you can expect to see
the F
1 generation emerge. After crossing the F
1 generation, the F
2 generation should appear within 2
weeks. You will have your choice of several phenotypes,
including wing structure, eye pigmentation, and body
coloration. We have even developed flightless strains,
so parents and teachers need not fear they will be
overrun with black clouds of fruit flies.
The right materials can make your work less complicated.
For instance, FlyNap® prepares flies more safely than
ever before. It anesthetizes young flies for up to 50
minutes, making handling for evaluation of expressed
phenotype easier. Supplies can be purchased separately
and in bulk. With 6 weeks of advance notice our
Drosophila Lab can also create customized F
1 crosses for
your project needs.
Commonly mistaken for insects, these relatives of
lobsters and crayfish are perfect for behavioral
investigations. The isopods most often used for
classroom studies are
Armadillidium vulgare (pill bug or
roly-poly) and
Porcellio laevis (sow bug).
Isopods can live in almost any plastic container,
provided there is a layer of moist soil covered with
dead leaves, twigs, mulch, or bark. A chunk of raw
potato or apple can serve double duty by providing both
food and moisture. The habitat must be kept moist at all
times—but not soaking wet—because the specimens obtain
the oxygen they need through gill-like structures found
on the bottom of their legs.
These organisms are not big fans of light, so your
terrarium or plastic container should be kept in a dimly
lit area. Under normal conditions isopods move about
slowly and feed casually; however, there is a noticeable
shift in their behavior when changes are made to
moisture, temperature, light, and touch stimuli.
These quick-developing
Brassica rapa plants are
excellent for use in many studies, particularly
examinations of plant life cycles, inheritance of stem
or leaf color, and maternal inheritance.
Wisconsin
Fast Plants® go from planted seed to seed in
about 40 to 55 days, depending on experimental
conditions. The plants grow quickly, emerging from a
specially formulated soil in 2 to 3 days, and are ready
for pollination at 11 to 14 days. They require a 24-hour
light source provided by cool, white fluorescent bulbs;
a constant water source; and consistent warm
temperatures.
There are several traits to choose from, including
expression of purple coloration (anthocyanin), leaf
hairs, yellow-green leaves, and dwarfism.
Many supplies are reusable and inexpensive to replace.
For project ideas and seed descriptions, visit our
resources and the Wisconsin
Fast Plants®
Web site.
Once you have tested your mastery of the scientific
method, you may wonder what to do with your living
specimen(s). Carolina provides living organisms for
educational purposes only, and we do not advocate the
release of specimens into local environments. We suggest
that organisms be maintained in your classroom, donated
to another classroom or your local natural science
center, or disposed of humanely as a last resort.
Read more
here.
Proper planning is the key to completing a successful
science project, and we hope the information provided
makes finding the right subject less complicated. Please
contact the Living Materials Department (800.334.5551)
if you have more questions.