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Separate and analyze amino acids using thin layer chromatography with this guided-inquiry kit. Students study 3 amino acids, then identify the components of an unknown mixture of amino acids using observations of the chromatograms and Rf factors. Kit includes materials to perform the activity 4 times with 10 groups of students.
Use thin layer chromatography to separate and identify amino acids
Students practice their thin layer chromatography technique on 3 known amino acid samples, reinforcing theory and technique. They calculate the retardation factor for each amino acid and make conclusions about the structure and properties of each amino acid based on its Rf value. Students then separate an unknown mixture of amino acids and use their previous data and observations to identify the amino acids present in the sample. Kit includes materials to perform the activity 4 times with 10 groups of students.
|0.1% Ninhydrin, 10 mL||2||Included|
|2-Propanol, 100%, 30 mL||2||Included|
|3 M Hydrochloric Acid, 30 mL||1||Included|
|Glacial Acetic Acid, 15 mL||2||Included|
|1:1 Butanol/Acetone, 30 mL||4||Included|
|Amino Acid Set||1||Included|
|Thin Layer Chromatography Plates||42||Included|
|Capillary Tube Vial||2||Included|
|Chromatography Chambers with Lids||12||Included|
|Digital Resource Card||1||Included|
|Teacher's Manual with Reproducible Student Guide||1||Included|
|Pencils||10||Needed, Not Included|
|Metric Rulers||10||Needed, Not Included|
|Flasks, 50 mL||4||Needed, Not Included|
|Flasks, 100 mL||1||Needed, Not Included|
|Graduated Cylinder, 100 mL (or smaller)||1||Needed, Not Included|
|Paper Towels||Needed, Not Included|
|Disposable Gloves||Needed, Not Included|
|Fume Hood||Needed, Not Included|
|Lab Oven or Hair Dryer (optional)||Needed, Not Included|