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Developed in cooperation with the DNA Learning Center, this advanced lab uses plasmid isolation and restriction analysis to illustrate forensic DNA typing. Students are provided with 2 samples of E. coli cells to represent blood samples from 2 suspects. Plasmid DNA is extracted using a miniprep procedure and cut with restriction enzymes, along with a DNA sample representing the evidence from a crime scene. The resulting “DNA fingerprints” of the suspects are compared for a match with the evidence.
AdvancedFor experienced high school and college classes; requires some technical skill.
Developed in cooperation with the DNA Learning Center, this advanced lab uses plasmid isolation and restriction analysis to illustrate forensic DNA typing. Students are provided with 2 samples of E. coli cells to represent blood samples from 2 suspects. Plasmid DNA is extracted using a miniprep procedure and cut with restriction enzymes, along with a DNA sample representing the evidence from a crime scene. The resulting “DNA fingerprints” of the suspects are compared for a match with the evidence. Materials are sufficient for 8 groups of 4 students. Foreign orders require special handling. Comes with perishables.
|E. coli Slant Cultures||2||Included|
|Restriction Buffer, 180 µL||1||Included|
|Restriction Enzyme Mixture, 85 µL||1||Included|
|RNase A, 250 µL, 5 µL/mL||1||Included|
|Evidence A DNA, 80 µL, 0.1 µg/µL||1||Included|
|Evidence B DNA, 80 µL, 0.1 µg/µL||1||Included|
|Luria Broth, 10 mL||4||Included|
|Glucose-Tris-EDTA, 3.5 mL||2||Included|
|Potassium Acetate-Acetic Acid Solution, 3.5 mL||2||Included|
|Isopropanol, 100%, 10 mL||2||Included|
|Ethanol, 95%, 15 mL||1||Included|
|Tris-EDTA Buffer, 3.5 mL||1||Included|
|Sodium Dodecyl Sulfate Solution, 10%, 1 mL||1||Included|
|0.22 N Sodium Hydroxide Solution, 8 mL||1||Included|
|Loading Dye, 1 mL||1||Included|
|TBE Electrophoresis Buffer, 20x, 200 mL||1||Included|
|Agarose, 5 g||1||Included|
|Sterile Distilled Water, 1 mL||1||Included|
|CarolinaBLU™ Final Stain, 400 mL||1||Included|
|CarolinaBLU™ Gel and Buffer Stain, 14 mL||1||Included|
|Microcentrifuge Tubes, 1.5 mL||130||Included|
|Digital Resource Instruction Card||1||Included|
|Teacher’s Manual with Reproducible Student Guide||1||Included|
|Water Bath, 37° C||Needed, Not Included|
|Water Bath, 60° C (optional)||Needed, Not Included|
|Microcentrifuge (that generates at least 2,300 x g)||Needed, Not Included|
|Gel Electrophoresis Chambers (with casting trays and well-forming combs)||Needed, Not Included|
|Electrophoresis Power Supplies||Needed, Not Included|
|White Light Transilluminator (for viewing gels)||Needed, Not Included|
|Micropipets (for 1- to 1,000-µL volumes)||Needed, Not Included|
|Masking Tape (for sealing gel casting tray)||Needed, Not Included|
|Racks (for 1.5-mL centrifuge tubes)||8||Needed, Not Included|
|Flask or Beaker, 1 L (for agarose)||Needed, Not Included|
|Beakers or Similar Containers, 600 mL (for ice)||8||Needed, Not Included|
|Beakers or Similar Containers, 600 mL (for waste, such as used pipets)||8||Needed, Not Included|
|Carboy or Container, 4 L or Larger (for electrophoresis buffer)||Needed, Not Included|
|Ice (preferably crushed)||Needed, Not Included|
|Distilled Water||Needed, Not Included|
|Permanent Markers||Needed, Not Included|
|Boiling Water Bath or Microwave Oven||Needed, Not Included|
|Autoclave or 10% Household Bleach Solution (for decontamination of bacterial waste before disposal)||Needed, Not Included|
|Disinfectant (10% household bleach solution, 70% ethanol, isopropanol, or other for cleaning lab surfaces)||Needed, Not Included|
|Paper Towels||Needed, Not Included|
|Camera (for recording results, optional)||Needed, Not Included|
Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.
Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.
At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.
The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.