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DNALC Forensic DNA Fingerprinting Kit with CarolinaBLU™ (with prepaid coupon)

Item # 211205

Developed in cooperation with the DNA Learning Center, this advanced lab uses plasmid isolation and restriction analysis to illustrate forensic DNA typing. Students are provided with 2 samples of E. coli cells to represent blood samples from 2 suspects. Plasmid DNA is extracted using a miniprep procedure and cut with restriction enzymes, along with a DNA sample representing the evidence from a crime scene. The resulting "DNA fingerprints" of the suspects are compared for a match with the evidence.

Price

$142.95

In stock and available to ship.

Advanced—For experienced high school and college classes; requires some technical skill.

Developed in cooperation with the DNA Learning Center, this advanced lab uses plasmid isolation and restriction analysis to illustrate forensic DNA typing. Students are provided with 2 samples of E. coli cells to represent blood samples from 2 suspects. Plasmid DNA is extracted using a miniprep procedure and cut with restriction enzymes, along with a DNA sample representing the evidence from a crime scene. The resulting “DNA fingerprints” of the suspects are compared for a match with the evidence. Materials are sufficient for 8 groups of 4 students. Kit includes a prepaid coupon to request perishable materials later at your convenience. Contact us or return the coupon to request delivery of perishables. Foreign orders require special handling.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

  • Excepted Quantity - This product is/contains a DOT regulated hazardous material in an excepted quantity. This does not incur a hazardous materials fee. Orders shipping to HI and AK are subject to review and may incur additional shipping charges.
  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.

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This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
E. coli Slant Cultures 2 Included
Restriction Buffer, 180 µL 1 Included
Restriction Enzyme Mixture, 85 µL 1 Included
RNase A, 250 µL, 5 µL/mL 1 Included
Evidence A DNA, 80 µL, 0.1 µg/µL 1 Included
Evidence B DNA, 80 µL, 0.1 µg/µL 1 Included
Luria Broth, 10 mL 4 Included
Glucose-Tris-EDTA, 3.5 mL 2 Included
Potassium Acetate-Acetic Acid Solution, 3.5 mL 2 Included
Isopropanol, 100%, 10 mL 2 Included
Ethanol, 95%, 15 mL 1 Included
Tris-EDTA Buffer, 3.5 mL 1 Included
Sodium Dodecyl Sulfate Solution, 10%, 1 mL 1 Included
0.22 N Sodium Hydroxide Solution, 8 mL 1 Included
Loading Dye, 1 mL 1 Included
TBE Electrophoresis Buffer, 20x, 200 mL 1 Included
Agarose, 5 g 1 Included
Sterile Distilled Water, 1 mL 1 Included
CarolinaBLU™ Final Stain, 400 mL 1 Included
CarolinaBLU™ Gel and Buffer Stain, 14 mL 1 Included
Microcentrifuge Tubes, 1.5 mL 130 Included
Staining Trays 8 Included
Transfer Pipets 100 Included
Disposable Gloves 16 Included
Inoculating Loops 4 Included
Digital Resource Instruction Card 1 Included
Teacher’s Manual with Reproducible Student Guide 1 Included
Water Bath, 37° C Needed, Not Included
Water Bath, 60° C (optional) Needed, Not Included
Microcentrifuge (that generates at least 2,300 x g) Needed, Not Included
Gel Electrophoresis Chambers (with casting trays and well-forming combs) Needed, Not Included
Electrophoresis Power Supplies Needed, Not Included
White Light Transilluminator (for viewing gels) Needed, Not Included
Micropipets (for 1- to 1,000-µL volumes) Needed, Not Included
Masking Tape (for sealing gel casting tray) Needed, Not Included
Racks (for 1.5-mL centrifuge tubes) 8 Needed, Not Included
Flask or Beaker, 1 L (for agarose) Needed, Not Included
Beakers or Similar Containers, 600 mL (for ice) 8 Needed, Not Included
Beakers or Similar Containers, 600 mL (for waste, such as used pipets) 8 Needed, Not Included
Carboy or Container, 4 L or Larger (for electrophoresis buffer) Needed, Not Included
Ice (preferably crushed) Needed, Not Included
Distilled Water Needed, Not Included
Permanent Markers Needed, Not Included
Boiling Water Bath or Microwave Oven Needed, Not Included
Autoclave or 10% Household Bleach Solution (for decontamination of bacterial waste before disposal) Needed, Not Included
Disinfectant (10% household bleach solution, 70% ethanol, isopropanol, or other for cleaning lab surfaces) Needed, Not Included
Paper Towels Needed, Not Included
Camera (for recording results, optional) Needed, Not Included

DNA Learning Center

Cutting Edge Science in your Classroom

Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.

Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.

At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.


About the DNA Learning Center

The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.

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