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Cancer diagnostics and treatments are on the front line of the biotechnological revolution. Your students get into the action by taking on the roles of researcher, clinician, and genetic counselor as they attempt to diagnose a family of patients. Using pedigrees and a simulated DNA-based diagnostic, students explore the genes linked to hereditary nonpolyposis colorectal cancer (HNPCC) in a fictitious family. Kit uses CarolinaBLU™ DNA stain and equips 32 students working in groups of 4.
Use heredity and biotechnology to explore colorectal cancer
Engaging students with a real-world impact
This lab provides students experience in drawing and interpreting a family pedigree in regards to a specific form of inherited colorectal cancer. The students then complete a DNA-based diagnostic test, using gel electrophoresis, of samples from the fictitious family to identify which members have inherited the cancer causing-mutation. After deciphering their results, students are tasked with taking on the role of a genetic counselor in charge of speaking with the family about the results.
Cancer diagnostics and treatments are on the front line of the biotechnological revolution. Researchers and clinicians are identifying new methods to identify the genes implicated in the cancer pathway and are applying the knowledge of those genes to develop targeted therapies. This laboratory exercise looks at genes linked to hereditary nonpolyposis colorectal cancer (HNPCC) and explores the connection between family medical history and testing for mutations in these genes.
Effortlessly incorporate into your classroom
This lab kit is designed to include all of the materials and background information you need to easily incorporate it into any course. The activity complements a study of the cell cycle and mitosis or a unit about genetic patterns of inheritance. Kit is designed for 32 students working in groups of 4. Requires 3 lab periods (4560 min) and 1 discussion session to complete. The gel electrophoresis portion of the lab is done using CarolinaBLU™ DNA stain. Kit ships with perishable materials included.
|DNA Ladder, 1 kb||8||Included|
|Normal Allele DNA (control), 25 µL||4||Included|
|Tumor DNA (control), 25 µL||4||Included|
|Heterozygote DNA (control), 25 µL||4||Included|
|Patient DNA (Marshall), 25 µL||4||Included|
|Patient DNA (Sara), 25 µL||4||Included|
|Patient DNA (Bob), 25 µL||4||Included|
|Patient DNA (Warren), 25 µL||4||Included|
|Patient DNA (Jane), 25 µL||4||Included|
|Patient DNA (Stan), 25 µL||4||Included|
|Patient DNA (Steven), 25 µL||4||Included|
|Patient DNA (Susan), 25 µL||4||Included|
|Patient DNA (Claire), 25 µL||4||Included|
|Agarose, 6 g||1||Included|
|TBE Buffer, 20x, 200 mL||1||Included|
|CarolinaBLU™ Final Stain, 250 mL||1||Included|
|CarolinaBLU™ Gel Stain, 7 mL||1||Included|
|Teacher’s Manual with Reproducible Student Guide||1||Included|
|Container for Buffer||Needed, Not Included|
|Aluminum Foil||Needed, Not Included|
|Distilled or Deionized Water||Needed, Not Included|
|Graduated Cylinder, 1 L||Needed, Not Included|
|Flask, 1 L||Needed, Not Included|
|Horizontal Agarose Gel Electrophoresis System||Needed, Not Included|
|Power Supply for Electrophoresis System||Needed, Not Included|
|Microwave, Hot Plate, or Boiling Water Bath||Needed, Not Included|
|Water Bath, 60° C||Needed, Not Included|
|Hot Pad or Glove||Needed, Not Included|
|Micropipets (2 to 20 µL, 1 or 2 per gel being run)||Needed, Not Included|
|Micropipet Tips||Needed, Not Included|
|Permanent Marker||Needed, Not Included|
|Racks for Samples||Needed, Not Included|
|White-Light Transilluminator||Needed, Not Included|
|Microcentrifuge (optional)||Needed, Not Included|
|Camera and Any Needed Filters (optional)||Needed, Not Included|