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HNPCC: Detecting Inherited Forms of Cancer GelGreen™ Kit

Item # 214821 Exclusive

Cancer diagnostics and treatments are on the front line of the biotechnological revolution. Your students get into the action by taking on the roles of researcher, clinician, and genetic counselor as they attempt to diagnose a family of patients. Using pedigrees and a simulated DNA-based diagnostic, students explore the genes linked to hereditary nonpolyposis colorectal cancer (HNPCC) in a fictitious family. Kit uses GelGreen™ DNA stain and equips 32 students working in groups of 4.

Price

$154.00

In stock and available to ship.

Use heredity and biotechnology to explore colorectal cancer

  • Create a real-world connection
  • Construct accurate pedigrees across multiple generations
  • Perform genetic testing using gel electrophoresis
  • Explore the ethical dilemmas associated with genetic testing

Engaging students with a real-world impact
This lab provides students experience in drawing and interpreting a family pedigree in regards to a specific form of inherited colorectal cancer. The students then complete a DNA-based diagnostic test, using gel electrophoresis, of samples from the fictitious family to identify which members have inherited the cancer causing-mutation. After deciphering their results, students are tasked with taking on the role of a genetic counselor in charge of speaking with the family about the results.

Biotechnological revolution
Cancer diagnostics and treatments are on the front line of the biotechnological revolution. Researchers and clinicians are identifying new methods to identify the genes implicated in the cancer pathway and are applying the knowledge of those genes to develop targeted therapies. This laboratory exercise looks at genes linked to hereditary nonpolyposis colorectal cancer (HNPCC) and explores the connection between family medical history and testing for mutations in these genes.

Effortlessly incorporate into your classroom
This lab kit is designed to include all of the materials and background information you need to easily incorporate it into any course. The activity complements a study of the cell cycle and mitosis or a unit about genetic patterns of inheritance. Kit is designed for 32 students working in groups of 4. Requires 3 lab periods (45–60 min) and 1 discussion session to complete. The gel electrophoresis portion of the lab is done using GelGreen™ DNA stain. Kit ships with perishable materials included.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

  • This item is only available from Carolina Biological Supply Company.
  • This item contains living or perishable material and ships via 2nd Day or Overnight delivery to arrive on a date you specify during Checkout. To ensure freshness during shipping, a Living Materials Fee may apply to orders containing these items.
  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.

Product Families Featuring This Item:

 
Components Qty Included?
DNA Ladder, 1 kb 8 Included
Normal Allele DNA (control), 25 µL 4 Included
Tumor DNA (control), 25 µL 4 Included
Heterozygote DNA (control), 25 µL 4 Included
Patient DNA (Marshall), 25 µL 4 Included
Patient DNA (Sara), 25 µL 4 Included
Patient DNA (Bob), 25 µL 4 Included
Patient DNA (Warren), 25 µL 4 Included
Patient DNA (Jane), 25 µL 4 Included
Patient DNA (Stan), 25 µL 4 Included
Patient DNA (Steven), 25 µL 4 Included
Patient DNA (Susan), 25 µL 4 Included
Patient DNA (Claire), 25 µL 4 Included
Agarose, 6 g 1 Included
TBE Buffer, 20x, 200 mL 1 Included
Staining Trays 8 Included
Sodium Chloride, 5 M, 8 mL 1 Included
GelGreen™ Gel Stain, 100 µL 1 Included
Gloves 16 Included
Teacher’s Manual with Reproducible Student Guide 1 Included
Container for Buffer Needed, Not Included
Aluminum Foil Needed, Not Included
Distilled or Deionized Water Needed, Not Included
Graduated Cylinder, 1 L Needed, Not Included
Flask, 1 L Needed, Not Included
Horizontal Agarose Gel Electrophoresis System Needed, Not Included
Power Supply for Electrophoresis System Needed, Not Included
Microwave, Hot Plate, or Boiling Water Bath Needed, Not Included
Water Bath, 60° C Needed, Not Included
Hot Pad or Glove Needed, Not Included
Micropipets (2 to 20 µL, 1 or 2 per gel being run) Needed, Not Included
Micropipet Tips Needed, Not Included
Permanent Marker Needed, Not Included
Racks for Samples Needed, Not Included
Blue-Light or UV Transilluminator Needed, Not Included
Microcentrifuge (optional) Needed, Not Included
Camera and Any Needed Filters (optional)  Needed, Not Included

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