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Restriction Enzyme and DNA 8-Station Kit with CarolinaBLU™

Item # 211185
Restriction Enzyme and DNA 8-Station Kit with CarolinaBLU™ is rated 5.0 out of 5 by 1.
  • y_2017, m_7, d_19, h_2
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This kit features our Instant Enzymes and Instant DNA. No more pipetting errors when adding enzyme to DNA; no more freezer storage or frozen samples; no more next day and ice pack shipping charges. The stable Instant DNA is simply rehydrated with distilled or deionized water. Kit contains sufficient materials for 8 teams of students.

Price

$120.00

In stock and available to ship.

Advanced - For experienced high school and college classes; requires some technical skill.

This kit features our Instant Enzymes and Instant DNA. No more pipetting errors when adding enzyme to DNA; no more freezer storage or frozen samples; no more next day and ice pack shipping charges. The stable Instant DNA is simply rehydrated with distilled or deionized water. After hydration, DNA is added to the tubes containing the Instant Enzymes (BamHI, EcoRI, and HindIII) and incubated at 37° C. After incubation, the DNA samples are electrophoresed on an agarose gel and stained. Kit contains sufficient materials for 8 teams of students. Kit also includes 2 bonus dry labs: "DNA Goes to the Races" and "DNA Scissors."

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.

Product Families Featuring This Item:

 

The kit contains sufficient materials for 8 teams of students.

Components Qty Included?
DNA and Restriction Enzymes Included
TAE Buffer, 100 mL 1 Included
Loading Dye, 100 µL 6 Included
CarolinaBLU™ Final Staining Solution, 250 mL 1 Included
CarolinaBLU™ Gel Stain, 7 mL 1 Included
Agarose, 3.2 g 1 Included
Microcentrifuge Tubes Included
Staining Trays 6 Included
Disposable Gloves 4 Included
Dry Labs 2 Included
Foam Microcentrifuge Rack 1 Included
Reproducible Student Guide 1 Included
Teacher Instructions 1 Included
Micropipettors Needed, Not Included
Gel Chamber Needed, Not Included
Water Bath (37° C) Needed, Not Included
Gel Visualization System Needed, Not Included
Power Supply Needed, Not Included
Rated 5 out of 5 by from Pleased with Purchase I had done ethidium bromide staining for years before I started teaching. That required a UV-light box, a UV-filter/camera set up, UV-face shield, etc. So being able to do this as a high school lab without having to have all the other equipment made this purchase very attractive to me. (Not to mention that your high schoolers don't have to work with a mutagen!) Anyway, this kit worked very well. It was a little disconcerting that the bromophenol blue band from the loading dye dissipated into the Carolina BLU-treated gel and running buffer. So I did not have a sign to watch for to turn off the electrophoresis. Do not panic if you cannot see any bands when you add the final stain for 20 minutes. I didn't either but as I destained with distilled water, they appeared clearly. Destaining was a bit inconvenient in the time that it took, but I was just impatient to see the results. I had good photography results by wrapping the gels in plastic wrap and laying on a color-flatbed scanner. Overall, I am well pleased with this purchase.
Date published: 2010-11-10
  • y_2017, m_7, d_19, h_2
  • bvseo_bulk, prod_bvrr, vn_bulk_1.0.0-hotfix-1
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_211185, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_carolina
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 11ms
  • REVIEWS, PRODUCT