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This kit provides data that allow students to assemble a map of cutting sites for ApaI, Eco0109I, and HindIII on the genome of the lambda virus. This is accomplished by digesting lambda DNA with the 3 restriction enzymes alone and in combination. A restriction map can be generated by using information gained from the electrophoresis of the lambda restriction fragments. Kit comes with perishables.
AdvancedFor experienced high school and college classes; requires some technical skill.
This kit provides data that allow students to assemble a map of cutting sites for ApaI, Eco0109I, and HindIII on the genome of the lambda virus. This is accomplished by digesting lambda DNA with the 3 restriction enzymes alone and in combination. A restriction map can be generated by using information gained from the electrophoresis of the lambda restriction fragments. Kit materials are sufficient for 6 teams of students or 6 separate instructor demonstrations. Kit ships with perishable materials.
|Lambda DNA, 0.4 µg/µL, 50 µL||6||Included|
|Restriction Buffer, 10x, 500 µL||1||Included|
|Loading Dye, 10x, 100 µL||6||Included|
|Electrophoresis Buffer, 150 mL||1||Included|
|CarolinaBLU™ Gel Stain, 7 mL||1||Included|
|CarolinaBLU™ Final Stain, 250 mL||1||Included|
|Agarose, 4 g||1||Included|
|Semi-Logarithmic Graph Paper||24||Included|
|37° C and 35° C Water Baths||Needed, Not Included|
|Gel Electrophoresis Chambers||Needed, Not Included|
|Power Supplies||Needed, Not Included|
|Gel Visualization Equipment (white light)||Needed, Not Included|
|Micropipettor||Needed, Not Included|
Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.
Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.
At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.
The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.