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A complete kit for conducting 6 Southern hybridization experiments using 7.5 x 10-cm membranes. Students map the location of a sequence within the bacteriophage lambda chromosome.
Advanced - For experienced high school and college classes; requires some technical skill.
A complete kit for conducting 6 Southern hybridization experiments using 7.5 x 10-cm membranes. Students map the location of a sequence within the bacteriophage lambda chromosome. Requires extended lab periods (call our Biotechnology Department for specifics). Kit comes with perishable materials. Note: Foreign orders require special handling.
|NBT/BCIP Color Development Solution||Included|
|Streptavidin-Alkaline Phosphatase Conjugate||Included|
|2x Denaturization Buffer||1||Included|
|2x Neutralization Buffer||2||Included|
|20x SSC Buffer||3||Included|
|Sodium Dodecyl Sulfate, 10%||1||Included|
|Buffer for Detection Steps||2||Included|
|Whatman Paper, 3 mm||8||Included|
|Agarose||Needed, Not Included|
|TBE Buffer||Needed, Not Included|
|Electrophoresis Equipment||Needed, Not Included|
|Gel Photography Equipment||Needed, Not Included|
|Oven (7080° C)||Needed, Not Included|
|Water Bath (50° C)||Needed, Not Included|
|Containers (for wash steps and transfer)||Needed, Not Included|
|Transparent Rulers||Needed, Not Included|