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Use methylation-sensitive enzymes to explore epigeneticsheritable changes in gene expressionthat affect flowering in Arabidopsis. Using techniques such as DNA extraction, restriction enzyme digest, PCR, gel electrophoresis, and bioinformatics, students learn the pivotal role DNA methylation plays in gene expression. Each kit contains materials for 25 DNA extractions and PCR reactions and materials for electrophoresis using GelGreen™.
AdvancedFor experienced high school and college classes; requires some technical skill.
|Arabidopsis Seed Pack from Ler (Landsberg erecta) Wild Type||1||Included|
|Arabidopsis Seed Pack from fwa-1 (flowering wageningen) Mutant||1||Included|
|FWA Primer/Loading Dye Mix, 700 µL||1||Included|
|McrBc Buffer, 10x, 200 µL||1||Included|
|McrBc Enzyme, 10 U/µL, 20 µL||1||Included|
|Guanosine 5′–Triphosphate (GTP), 100 mM, 15 µL||1||Included|
|BSA, 10 mg/mL, 30 µL||1||Included|
|Sterile Distilled Water, 1 mL||1||Included|
|RNAse Solution, 5 mg/mL, 250 µL||1||Included|
|Potting Soil, Bag||1||Included|
|pBR322/BstNI Markers, 0.075 µg/µL, 130 µL||1||Included|
|Ready-to-Go™ PCR Beads, 0.2-mL Tubes||25||Included|
|Nuclei Lysis Solution, 12 mL||1||Included|
|Protein Precipitation Solution, 3.5 mL||1||Included|
|Isopropanol, 100%, 20 mL||2||Included|
|Ethanol, 70%, 30 mL||1||Included|
|DNA Rehydration Buffer, 2.5 mL||1||Included|
|Mineral Oil, 5 mL||1||Included|
|Agarose, 4 g||2||Included|
|20x TBE Buffer, 150 mL||1||Included|
|GelGreen™ Nucleic Acid Gel Stain, 100 µL||1||Included|
|NaCl, 5 M, 8 mL||1||Included|
|Planting Flat and Tray||1||Included|
|Plastic Dome Lid||1||Included|
|Teacher’s Manual with Reproducible Student Guide||1||Included|
|Tweezers, Scissors, or Scalpel||Needed, Not Included|
|Water Baths/Heating Blocks (65° C and 37° C)||Needed, Not Included|
|Micropipets and Tips, 1 to 1,000 µL||Needed, Not Included|
|Microcentrifuge||Needed, Not Included|
|Microcentrifuge Tubes, 1.5 mL||32||Needed, Not Included|
|Microcentrifuge Tube Racks||8||Needed, Not Included|
|Thermal Cycler, Programmable||1||Needed, Not Included|
|Electrophoresis Chambers||Needed, Not Included|
|Electrophoresis Power Supplies||Needed, Not Included|
|Container with Ice||Needed, Not Included|
|Computer with Internet Access||Needed, Not Included|
|Microwave, Hot Plate, or Boiling Water||Needed, Not Included|
|Vortexer (optional)||Needed, Not Included|
Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.
Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.
At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.
The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.
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