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Mitochondrial DNA Polymorphisms in Human Evolution DNA Extraction, Amplification, and Electrophoresis Kit with Ethidium Bromide Stain and 0.2-mL Tubes (with prepaid coupon)

Item # 211237A Exclusive

Advanced—For experienced high school and college classes; requires some technical skill.
Students use a classroom-friendly protocol involving saline mouthwash or a hair sample and Chelex® extraction to obtain a sample of their own DNA. This kit then has students amplify and sequence a 440-nucleotide segment of a hypervariable region of the mitochondrial chromosome. Kit includes enough materials for 25 students to perform DNA extraction, amplification, and electrophoresis with ethidium bromide.

Price

$220.00

In stock and available to ship.

Advanced—For experienced high school and college classes; requires some technical skill.

Generate excitement by having your students amplify their own mitochondrial DNA for sequencing!

  • Uses a simple, robust protocol ideal for the classroom
  • Enlivens discussions on the molecular basis of heredity and evolution
  • Provides a basic version of the lab that is used in forensics and the analysis of ancient DNA samples
  • Teaches real-world applications of PCR
  • Includes online resources to enhance the learning experience

Students use safe saline mouthwash or hair and Chelex® extraction to obtain a sample of their own DNA. Then they amplify a 440-nucleotide segment of a hypervariable region of the mitochondrial chromosome, which contains numerous single nucleotide polymorphisms (SNPs). After gel electrophoresis confirms amplification, the student samples may be sent for sequencing (the cost of sequencing is not included in the price of the kit). Students then compare their SNPs to ancient hominids and to people from different world populations to discover patterns of DNA variation and to analyze theories of human evolution.

Kit contains instructions and materials for 25 students to perform DNA extraction, amplification, and electrophoresis (staining with ethidium bromide). Note: Kit includes a prepaid coupon to request perishable materials later at your convenience. Contact us or return the coupon to request delivery of perishable materials.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

  • This item is only available from Carolina Biological Supply Company.
  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.

Product Families Featuring This Item:

 

This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
mtDNA Primer/Loading Dye Mix, 700 µL 1 Included
pBr322/BstNI Markers, 0.075 µg/µL, 130 µL 1 Included
Proteinase K, 100 µg/mL, 5 mL 1 Included
Chelex® 100 Resin, 1.5 g 1 Included
PCR Tubes Containing Ready-to-Go™ Beads (0.2-mL capacity) 25 Included
Mineral Oil, 5 mL 1 Included
Agarose, 5 g 1 Included
TBE Buffer, 150 mL 1 Included
Ethidium Bromide, 1 µg/mL, 250 mL 1 Included
Latex Gloves 4 Included
Staining Trays 6 Included
Teacher's Manual with Reproducible Student Guide 1 Included
Aluminum Foil Needed, Not Included
Beakers Containing Ice Needed, Not Included
Programmable DNA Thermal Cycler Needed, Not Included
Electrophoresis Chambers Needed, Not Included
Electrophoresis Power Supplies Needed, Not Included
Forceps Needed, Not Included
Laboratory Markers (1 per student) Needed, Not Included
Micropipets and Tips, 100–1,000 µL Needed, Not Included
Micropipets and Tips, 1–10 µL or 1–20 µL Needed, Not Included
Microcentrifuge Tube Racks Needed, Not Included
Microcentrifuge Tubes, 1.5 mL Needed, Not Included
Paper Cups Needed, Not Included
Razor Blade or Scalpel Needed, Not Included
Saline Solution, 0.9% Needed, Not Included
Boiling Water Baths Needed, Not Included
Water Baths, 37° and 65° C Needed, Not Included
UV Transilluminator (for ethidium bromide stain) Needed, Not Included
Vortexer (optional) Needed, Not Included
Centrifuge for 15-mL Tubes (minimum 500 x g; optional but useful) Needed, Not Included
Centrifuge for 1.5-mL Tubes (optional but useful) Needed, Not Included
Camera (optional) Needed, Not Included

DNA Learning Center

Cutting Edge Science in your Classroom

Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.

Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.

At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.


About the DNA Learning Center

The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.

Correlations to State Standards

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