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A truly teacher- and student-friendly approach to conducting human genetics studies in your classroom. This high-quality kit gives your students the opportunity to determine their own genotype by looking at a 300-nucleotide Alu insertion (PV92). The experiment is set up to ensure students make real-world connections between population genetics, evolution, and much more. Kit includes enough materials for 25 students to perform DNA extraction, amplification, and electrophoresis with CarolinaBLU™ stain.
AdvancedFor experienced high school and college classes; requires some technical skill.
Students use safe saline mouthwash or hair and Chelex® extraction to obtain a sample of their own DNA. Then they use polymerase chain reaction (PCR) to amplify a 300-nucleotide Alu insertion into an intron of the H-cadherin gene. Gel electrophoresis separates the 2 alleles, and each student determines his/her own genotype. The experiment provides an introduction to human population genetics as participants learn to score genotypes and calculate genotype and allele frequencies.
Kit contains instructions, a CD-ROM, and materials for 25 students to perform DNA extraction, amplification, and electrophoresis (staining with CarolinaBLU™). Note: Kit is shipped with perishable materials.
|PV92B Primer/Loading Dye Mix, 700 µL||1||Included|
|pBr322/B s t NI Markers, 0.075 µg/µL, 130 µL||1||Included|
|Proteinase K, 100 µg/mL, 5 mL||1||Included|
|Chelex® Resin, 1.5 g||1||Included|
|PCR Tubes Containing Ready-to-Go-Beads™ (0.2-mL capacity)||25||Included|
|Mineral Oil, 5 mL||1||Included|
|Agarose, 5 g||1||Included|
|TBE, 150 mL||1||Included|
|CarolinaBLU™ Final Stain, 250 mL||1||Included|
|CarolinaBLU™ Gel and Buffer Stain, 7 mL||1||Included|
|Teacher's Manual with Reproducible Student Guide||1||Included|
|Saline Solution, 0.9% (10 mL per student)||Needed, Not Included|
|Micropipets and Tips (1 µL to 1,000 µL)||Needed, Not Included|
|Microcentrifuge Tubes, 1.5 mL (2 per student)||Needed, Not Included|
|Microcentrifuge Tube Racks||Needed, Not Included|
|Microcentrifuge for 1.5-mL Tubes||Needed, Not Included|
|PCR Tubes, 0.2 mL or 0.5 mL (1 per student)||Needed, Not Included|
|Tube Adapters (for microcentrifuge)||Needed, Not Included|
|Thermal Cycler||Needed, Not Included|
|Electrophoresis Chambers||Needed, Not Included|
|Electrophoresis Power Supplies||Needed, Not Included|
|Gel Staining Trays||Needed, Not Included|
|White Light Box (for CarolinaBLU™ stain)||Needed, Not Included|
|Paper Cup (1 per student)||Needed, Not Included|
|Permanent Markers||Needed, Not Included|
|Container of Crushed Ice||Needed, Not Included|
|Camera (optional)||Needed, Not Included|
|Boiling Water Bath (optional)||Needed, Not Included|
Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.
Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.
At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.
The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.