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Human Alu DNA Extraction and Amplification Kit with 0.2-mL Tubes (with perishables)

Item # 211230AP Exclusive

A truly teacher- and student-friendly approach to conducting human genetics studies in your classroom. This high-quality kit gives your students the opportunity to determine their own genotype by looking at a 300-nucleotide Alu insertion (PV92). The experiment is set up to ensure students make real-world connections between population genetics, evolution, DNA sequencing, and much more. Kit comes with enough materials for 25 students to perform the DNA extraction and amplification process.

Price

$200.00

In stock and available to ship.

Advanced—For experienced high school and college classes; requires some technical skill.

  • Simple, robust protocol is ideal for classroom use
  • Incorporates Hardy-Weinberg equilibrium and chi-square analysis
  • Helps demonstrate real-world applications of PCR
  • Discover how Alu insertions are used to track world population migrations
  • CD-ROM contains animations and video clips for classroom instruction

Students use safe saline mouthwash or hair and Chelex® extraction to obtain a sample of their own DNA. Then they use polymerase chain reaction (PCR) to amplify a 300-nucleotide Alu insertion into an intron of the H-cadherin gene. Gel electrophoresis separates the 2 alleles, and each student determines his/her own genotype. The experiment provides an introduction to human population genetics as participants learn to score genotypes and calculate genotype and allele frequencies.

Kit contains instructions, a CD-ROM, and materials for 25 students to perform DNA extraction and amplification. Kit does not include materials for electrophoresis. Note: Kit is shipped with perishable materials.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

  • This item is only available from Carolina Biological Supply Company.
  • This item contains living or perishable material and ships via 2nd Day or Overnight delivery to arrive on a date you specify during Checkout. To ensure freshness during shipping, a Living Materials Fee may apply to orders containing these items.
  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.
 

This item ships with living or perishable materials (all   items). Please select a delivery date during Checkout.

Components Qty Included?
PV92B Primer/Loading Dye Mix, 700 µL  1 Included
pBr322/B s t NI Markers, 0.075 µg/µL, 130 µL  1 Included
Proteinase K, 100 µg/mL, 5 mL  1 Included
Chelex® Resin, 1.5 g 1 Included
PCR Tubes Containing Ready-to-Go-Beads™ (0.2-mL capacity) 25 Included
Mineral Oil, 5 mL 1 Included
Alu CD-ROM 1 Included
Teacher's Manual with Reproducible Student Guide 1 Included
Saline Solution, 0.9% (10 mL per student) Needed, Not Included
Micropipets and Tips (1 µL to 1,000 µL) Needed, Not Included
Microcentrifuge Tubes, 1.5 mL (2 per student) Needed, Not Included
Microcentrifuge Tube Racks Needed, Not Included
Microcentrifuge for 1.5-mL Tubes Needed, Not Included
PCR Tubes, 0.2 mL or 0.5 mL (1 per student) Needed, Not Included
Tube Adapters (for microcentrifuge) Needed, Not Included
Thermal Cycler Needed, Not Included
Electrophoresis Chambers Needed, Not Included
Electrophoresis Power Supplies Needed, Not Included
Gel Staining Trays Needed, Not Included
UV Transilluminator (for ethidium bromide stain) Needed, Not Included
White Light Box (for CarolinaBLU™ stain) Needed, Not Included
Paper Cup (1 per student) Needed, Not Included
Permanent Markers Needed, Not Included
Container of Crushed Ice Needed, Not Included
Camera (optional) Needed, Not Included
Boiling Water Bath (optional) Needed, Not Included
 

DNA Learning Center

Cutting Edge Science in your Classroom

Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.

Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.

At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.


About the DNA Learning Center

The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.

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