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Using Highly Variable Polymorphisms in Forensic Biology and Population Genetics DNA Extraction, Amplification, and Electrophoresis Kit with CarolinaBLU™ and 0.2-mL Tubes (with prepaid coupon)

Item # 211235A Exclusive

A teacher- and student-friendly approach to introducing the role of PCR in forensics, this lab illustrates the use of DNA typing to identify individuals in court cases and disasters. It assays for variable numbers of tandem repeats (VNTR) polymorphisms, which are caused by short, repeated copies of a 16-nucleotide sequence at the pMCT118 locus. Kit comes with enough materials for 25 students to perform DNA extraction, amplification, and electrophoresis with CarolinaBLU™.

Price

$210.00

This item will ship on or about 12/22/16

Advanced—For experienced high school and college classes; requires some technical skill.

Introduce students to Hardy-Weinberg equilibrium and the concept of allele frequencies in populations

  • Provides an introduction to the use of PCR in forensics
  • Helps demonstrate real-world applications of PCR

This lab illustrates the use of DNA typing to identify individuals in court cases and disasters. It assays for variable numbers of tandem repeats (VNTR) polymorphisms, which are caused by short, repeated copies of a 16-nucleotide sequence at the pMCT118 locus. Differences in the number of repeated units produce longer and shorter alleles, which can be resolved by gel electrophoresis. Because the VNTR locus has more than 29 different alleles, a panel of student types shows a variety of different genotypes.

Use this kit to teach population genetics, Hardy-Weinberg equilibrium, and forensics techniques. The repeated structure of the VNTR polymorphism makes it more difficult to amplify, so this experiment greatly benefits from a “hot start,” in which 1 of the reagents is added during the first denaturing cycle.

Kit contains instructions and materials for 25 students to perform the DNA extraction, amplification, and electrophoresis (staining with CarolinaBLU™). Note: Kit includes a prepaid coupon to request perishable materials later at your convenience. Contact us or return the coupon to request delivery of perishable materials.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

  • This item is only available from Carolina Biological Supply Company.
  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.
 

This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
pMCT118 Primer/Loading Dye Mix, 700 µL 1 Included
pBr322/B s t NI Markers, 0.075 µg/µL, 130 µL 1 Included
Proteinase K, 100 µg/mL, 5 mL 1 Included
Chelex® Resin, 1.5 g 1 Included
Mineral Oil, 5 mL 1 Included
PCR Tubes Containing Ready-to-Go-Beads™ (0.2-mL capacity) 25 Included
Agarose, 5 g 1 Included
TBE, 150 mL 1 Included
CarolinaBLU™ Final Stain, 250 mL 1 Included
CarolinaBLU™ Gel and Buffer Stain, 7 mL 1 Included
Latex Gloves 4 Included
Staining Trays 6 Included
Teacher's Manual with Reproducible Student Guide 1 Included
Saline Solution, 0.9% (10 mL per student) Needed, Not Included
Micropipets and Tips (1 µL to 1,000 µL) Needed, Not Included
Microcentrifuge Tubes, 1.5 mL (2 per student) Needed, Not Included
Microcentrifuge Tube Racks Needed, Not Included
Microcentrifuge for 1.5-mL Tubes Needed, Not Included
PCR Tubes, 0.2 mL or 0.5 mL (1 per student) Needed, Not Included
Tube Adapters (for microcentrifuge) Needed, Not Included
Thermal Cycler Needed, Not Included
Electrophoresis Chambers Needed, Not Included
Electrophoresis Power Supplies Needed, Not Included
Gel Staining Trays Needed, Not Included
White Light Box (for CarolinaBLU™ stain) Needed, Not Included
Paper Cup (1 per student) Needed, Not Included
Permanent Markers Needed, Not Included
Container of Crushed Ice Needed, Not Included
Camera (optional) Needed, Not Included
Boiling Water Bath (optional) Needed, Not Included

DNA Learning Center

Cutting Edge Science in your Classroom

Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.

Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.

At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.


About the DNA Learning Center

The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.

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