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A teacher- and student-friendly approach to introducing the role of PCR in forensics, this lab illustrates the use of DNA typing to identify individuals in court cases and disasters. It assays for variable numbers of tandem repeats (VNTR) polymorphisms, which are caused by short, repeated copies of a 16-nucleotide sequence at the pMCT118 locus. Kit comes with enough materials for 25 students to perform DNA extraction, amplification, and electrophoresis with CarolinaBLU™.
AdvancedFor experienced high school and college classes; requires some technical skill.
Introduce students to Hardy-Weinberg equilibrium and the concept of allele frequencies in populations
This lab illustrates the use of DNA typing to identify individuals in court cases and disasters. It assays for variable numbers of tandem repeats (VNTR) polymorphisms, which are caused by short, repeated copies of a 16-nucleotide sequence at the pMCT118 locus. Differences in the number of repeated units produce longer and shorter alleles, which can be resolved by gel electrophoresis. Because the VNTR locus has more than 29 different alleles, a panel of student types shows a variety of different genotypes.
Use this kit to teach population genetics, Hardy-Weinberg equilibrium, and forensics techniques. The repeated structure of the VNTR polymorphism makes it more difficult to amplify, so this experiment greatly benefits from a “hot start,” in which 1 of the reagents is added during the first denaturing cycle.
Kit contains instructions and materials for 25 students to perform the DNA extraction, amplification, and electrophoresis (staining with CarolinaBLU™). Note: Kit is shipped with perishable materials.
|pMCT118 Primer/Loading Dye Mix, 700 µL||1||Included|
|pBr322/B s t NI Markers, 0.075 µg/µL, 130 µL||1||Included|
|Proteinase K, 100 µg/mL, 5 mL||1||Included|
|Chelex® Resin, 1.5 g||1||Included|
|Mineral Oil, 5 mL||1||Included|
|PCR Tubes Containing Ready-to-Go-Beads™ (0.2-mL capacity)||25||Included|
|Agarose, 5 g||1||Included|
|TBE, 150 mL||1||Included|
|CarolinaBLU™ Final Stain, 250 mL||1||Included|
|CarolinaBLU™ Gel and Buffer Stain, 7 mL||1||Included|
|Teacher's Manual with Reproducible Student Guide||1||Included|
|Saline Solution, 0.9% (10 mL per student)||Needed, Not Included|
|Micropipets and Tips (1 µL to 1,000 µL)||Needed, Not Included|
|Microcentrifuge Tubes, 1.5 mL (2 per student)||Needed, Not Included|
|Microcentrifuge Tube Racks||Needed, Not Included|
|Microcentrifuge for 1.5-mL Tubes||Needed, Not Included|
|PCR Tubes, 0.2 mL or 0.5 mL (1 per student)||Needed, Not Included|
|Tube Adapters (for microcentrifuge)||Needed, Not Included|
|Thermal Cycler||Needed, Not Included|
|Electrophoresis Chambers||Needed, Not Included|
|Electrophoresis Power Supplies||Needed, Not Included|
|Gel Staining Trays||Needed, Not Included|
|White Light Box (for CarolinaBLU™ stain)||Needed, Not Included|
|Paper Cup (1 per student)||Needed, Not Included|
|Permanent Markers||Needed, Not Included|
|Container of Crushed Ice||Needed, Not Included|
|Camera (optional)||Needed, Not Included|
|Boiling Water Bath (optional)||Needed, Not Included|
Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.
Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.
At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.
The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.