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Drawing real-world connections in your classroom is a surefire way to create unique learning experiences. This lab activity is geared at giving your students hands-on experience with the concepts of evolution and antibiotic resistance in a completely safe environment. Using a bacterial population of E. coli and observing its response to ampicillin, your students can visualize the process of natural selection right in your classroom.
Looking for an engaging activity that blends the topic of evolution with the real-world issue of antibiotic resistance? In this experiment, students observe firsthand the evolution of a bacterial population’s resistance to antibiotics over generations. It gives students the opportunity to use sterile technique, perform serial dilutions, plate bacteria, and understand the process of natural selection. This 8-Station Kit is designed for 8 groups of 2 to 4 students. Note: Includes perishable materials.
|MM294 Slant Culture||1||Included|
|MM294/pAMP Slant Culture||1||Included|
|Ampicillin Vial, 100 mg/mL, 4 mL||1||Included|
|Ampicillin Vial, 100 mg/mL, 200 µL||8||Included|
|Glass Beads, Pack||3||Included|
|Sterile Petri Dishes||40||Included|
|Sterile LB Agar, Bottle, 400 mL||2||Included|
|Sterile LB Broth, 50 mL||8||Included|
|Disposable Inoculating Loops||20||Included|
|Nichrome Wire Inoculating Loop||1||Included|
|Teacher’s Manual with Reproducible Student Guide||1||Included|
|Incubator, 37° C||Needed, Not Included|
|Pipets or Measuring Device, 1 and 2 mL||Needed, Not Included|
|Micropipets for 10-, 20-, 22-, and 100-µL Volumes||Needed, Not Included|
|Micropipet Tips||Needed, Not Included|
|Microwave or Boiling-Water Bath||Needed, Not Included|
|Bunsen Burners||Needed, Not Included|
|Waste Containers||Needed, Not Included|
|Household Bleach or Ethanol||Needed, Not Included|
|Permanent Markers||Needed, Not Included|
|Culture Tube Racks||Needed, Not Included|
|Hot Pad or Gloves||Needed, Not Included|
|Microcentrifuge Tubes, Sterile||Needed, Not Included|
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