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Using a Single Nucleotide Polymorphism (SNP) to Predict Bitter Tasting Ability Kit

10 Items
$31.50 - $240.00 View Details

This kit explores the molecular basis of the inherited ability to taste the bitter chemical phenylthiocarbamide (PTC). Students determine their ability to taste PTC using taste paper. They then use safe saline mouthwash and Chelex® extraction to obtain a sample of their own DNA and amplify a 221-nucleotide region of the PTC taste receptor gene.

 
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This kit explores the molecular basis of the inherited ability to taste the bitter chemical phenylthiocarbamide (PTC). Students determine their ability to taste PTC using taste paper. They then use safe saline mouthwash and Chelex® extraction to obtain a sample of their own DNA and amplify a 221-nucleotide region of the PTC taste receptor gene. The 2 alleles differ by a single nucleotide, so restriction digestion of the amplified product followed by gel electrophoresis effectively differentiates the 2 alleles. One allele strongly correlates to the ability to taste PTC. After scoring their SNP genotypes, students determine how well the SNP genotypes actually correlate to tasting.

Each kit includes materials for 25 PCR reactions and restriction digests, and a PTC CD-ROM. CD-ROM includes animations and additional resources.

** Kit and perishable components will ship separately and may arrive on different days.

 
 
 
 
 

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