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Detecting Genetically Modified Food by PCR

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Detecting Genetically Modified Food by PCR is rated 5.0 out of 5 by 2.
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$200.00 - $275.00 View Details

Explore a modern application of genetic engineering through this exciting laboratory activity. This activity investigates whether the soy or corn ingredients in various processed foods contain a genetic modification. Students isolate DNA from wild-type and GM plant material (provided controls), and from food products of their choice. They use the extracted DNA as a template in 2 separate PCR reactions run under the same conditions. Each group works with a plant control and a food product.

 
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Advanced - For experienced high school and college classes; requires some basic technical skill.

Explore a modern application of genetic engineering through this exciting laboratory activity. Many commercial crops are genetically modified (GM) to include a transferred gene (transgene) for herbicide resistance. This activity investigates whether the soy or corn ingredients in various processed foods contain a genetic modification. Students isolate DNA from wild-type and GM plant material (provided controls), and from food products of their choice. They use the extracted DNA as a template in 2 separate PCR reactions run under the same conditions. One reaction serves as a positive control to amplify a portion of the tubulin gene found in all plants, and the other assays for genetic modification. Each group works with a plant control and a food product. Each kit includes materials for 25 PCR reactions. Note: Order the kit with the perishable materials included (kit and perishable components ship separately and may arrive on different days) or with a prepaid coupon to request perishables later at your convenience. Contact us or return the coupon to request delivery of perishable materials. Order kit to arrive 1 month before performing lab.

 
 
 
 
Rated 5 out of 5 by from this is an excelelent kit to teach and demonstrate concepts at various levels I use this kit to demonstrate concepts of genetics to high school students. They enjoyed the experiment and learn a variety of concepts including the GMOs, DNA and genes and amplification and visualization of DNA.
Date published: 2015-11-03
Rated 5 out of 5 by from Best Procedure for Excellent Results For beginning biotechnology students, this kit has everything needed to extract DNA, amplify it with PCR, and get excellent results using electrophoresis. After many attempts using this kit, we found the key to obtain perfect gels. Run your thermocycler four times longer than instructed and use ethidium bromide as your stain. We have one group use edamama (soy) beans purchased at our local grocery, for a good source of a positive GMO food besides the soy leaf that comes in the kit as a positive control. Leave your finished gels in the ethidium bromide overnight for best stain results. Students enjoy this lab experiment and are always excited to see their results.
Date published: 2014-12-04
  • y_2017, m_6, d_27, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_1.0.0-hotfix-1
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_FAM_211366, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_carolina
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 6ms
  • REVIEWS, PRODUCT