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Mitochondrial DNA Polymorphisms in Human Evolution

13 Items ExclusiveOnline Only
0.0 - $235.00 View Details

Students use a classroom-friendly protocol involving saline mouthwash or a hair sample and Chelex® extraction to obtain a sample of their own DNA. This high-quality kit then has students amplify and sequence a 440-nucleotide segment of a hypervariable region of the mitochondrial chromosome. The materials and instructions in this kit are designed for use in the classroom to help guarantee success. Kit comes with enough materials for 25 students.

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Advanced— For experienced high school and college classes; requires some technical skill.

Generate excitement by having your students amplify their own mitochondrial DNA for sequencing!

  • Uses a simple, robust protocol ideal for the classroom
  • Enlivens discussions on the molecular basis of heredity and evolution
  • Provides a basic version of the lab that is used in forensics and the analysis of ancient DNA samples
  • Teaches real-world applications of PCR
  • Includes online resources to enhance the learning experience

Students use safe saline mouthwash or hair and Chelex® extraction to obtain a sample of their own DNA. Then they amplify a 440-nucleotide segment of a hypervariable region of the mitochondrial chromosome, which contains numerous single nucleotide polymorphisms (SNPs). After gel electrophoresis confirms amplification, the student samples may be sent for sequencing (the cost of sequencing is not included in the price of the kit). Students then compare their SNPs to ancient hominids and to people from different world populations to discover patterns of DNA variation and to analyze theories of human evolution.

Each kit contains instructions and materials for 25 students. Note: Order the kit with the perishable materials included or with a prepaid coupon to request perishables later at your convenience. Contact us or return the coupon to request delivery of perishable materials. Sold only to schools and businesses.

 
 
 
 
 
 
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