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Advanced--For experienced high school and college classes; requires some technical skill. Looking to build on your students’ classroom knowledge of DNA fingerprinting with a hands-on activity? By conducting a PCR reaction followed by a gel electrophoresis analysis, your students work together to identify the culprit of the crime. Materials for 28 PCR reactions and electrophoresis using CarolinaBLU.
Advanced--For experienced high school and college classes; requires some technical skill. Looking to build on your students’ classroom knowledge of DNA fingerprinting with a hands-on activity? This kit takes your forensics class to the next level by giving students the opportunity to analyze DNA samples from a (fictitious) crime scene. As students immerse themselves in attempting to identify the culprit, they learn more about DNA profiling and the role of tandem repeats in the analysis. They build on that knowledge by conducting a hands-on investigation of the samples using PCR amplification and gel electrophoresis using CarolinaBLU™ to help close the case. This kit is a great next step for bringing molecular techniques into your forensics classroom or an ideal investigation for engaging your biology classroom in a new way. Kit contains materials for 28 PCR reactions and for electrophoresis using CarolinaBLU™. Kit also includes a prepaid coupon to request perishable materials later at your convenience. Contact Carolina or return the coupon to request delivery of perishable materials. For students working in 6 groups of 4.
|DNA Sample 1: Victim Cheek Swab, 24 μL||1||Included|
|DNA Sample 2: Suspect #1, Trisha's Cheek Swab, 24 μL||1||Included|
|DNA Sample 3: Suspect #2, Neil's Cheek Swab, 24 μL||1||Included|
|DNA Sample 4: Saliva from Wine Glass, 24 μL||1||Included|
|DNA Sample 5: Skin Collected from the Victim's Fingernails, 24 μL||1||Included|
|DNA Sample 6: Positive Control, Known Genomic DNA, 24 μL||1||Included|
|Primer Mix with Loading Dye, 600 μL||1||Included|
|DNA Ladder with Loading Dye, 50 μL||1||Included|
|Ready-to-Go™ PCR Beads||28||Included|
|Sterile Distlled Water, 1 mL||1||Included|
|Agarose, 5 g||1||Included|
|TBE Electrophoresis Medium, 200 mL||1||Included|
|CarolinaBLU™ Final Stain, 250 mL||1||Included|
|Teacher's Manual and Reproducible Student Guide||1||Included|
|Distilled Water, 4.1 L||1||Needed, Not included|
|Electrophoresis Setups and Power Supplies||4||Needed, Not included|
|Mid-range Micropipets with Tips (20-200 μL range)||5||Needed, Not included|
|Low-range Micropipets with Tips (1-20 μL range)||5||Needed, Not included|
|Bottle or Carboy, 4 L||1||Needed, Not included|
|Flask, 500 mL||1||Needed, Not included|
|Thermocycler||1||Needed, Not included|
|Light Box||1||Needed, Not included|
|Waterproof Marking Pens||Needed, Not included|
|Masking Tape||Needed, Not included|
|Microcentrifuge Tube Racks||Needed, Not included|
|Gloves||Needed, Not included|
|Foil (for covering liquid agarose; optional)||Needed, Not included|
|PCR Tube Racks (optional)||Needed, Not included|
|Water Bath, 60°C (optional)||Needed, Not included|
|Photo Equipment (optional)||Needed, Not included|
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