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Genotype-Phenotype Connection Extraction and Amplification Kit (with prepaid coupon)

Item # 211217 Exclusive

Introduce your students to a new model organism while building their inquiry skills and advanced molecular techniques. Students use the model microbe Halobacterium sp. NRC-1 to perform DNA extractions, PCR, and gel electrophoresis. Kit contains teacher’s manual and materials for 25 DNA extractions and PCR reactions. Note: Kit also includes a coupon to request shipment of perishable materials later at your convenience (and at no extra charge).

Price

$204.95

In stock and available to ship.

Looking for a great way to introduce your students to a new model organism while building their inquiry skills and advanced molecular techniques? In this activity, students use the model microbe Halobacterium sp. NRC-1 to perform deoxyribonucleic acid (DNA) extractions, polymerase chain reactions (PCR), and gel electrophoresis. The activity is designed in inquiry format to allow students to observe and experience the Archaeon and extreme halophile (salt-loving) microorganism, Halobacterium sp. NRC-1, along with a stable mutant derivative, Halobacterium sp. KBT-1. Students identify the phenotypic differences between these colonies and use PCR to analyze DNA from either a mutant (KBT-1), a wild type (NRC-1), or a sectored colony, further cementing the genotype-phenotype connection. Kit contains teacher’s manual with reproducible student guide along with materials for 25 DNA extractions and PCR reactions.

  • This item is only available from Carolina Biological Supply Company.
  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses.

Product Families Featuring This Item:

  • Save 10% off your online order of $250 or more! To receive the discount, just enter promo code SUMMER during Checkout.
 

This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
Halobacterium sp. KBT-1 (mutant) 2 Included
Halobacterium sp. NRC-1 (wild type) 3 Included
Primer-Loading Dye Mix 1 Included
1-kb Ladder/DNA Marker, 30 μL 4 Included
Sterile Water, 20 mL 2 Included
PCR Tubes Containing Ready-To-Go-Beads™ (0.2 mL capacity) 25 Included
Teacher's Manual and Reproducible Student Guide 1 Included
Pipets Needed, Not included
Resealable Plastic Bags or Containers Needed, Not included
Permanent Fine-tip Markers Needed, Not included
Microcentrifuge (with adapters for 0.2 mL tubes) Needed, Not included
Ice Needed, Not included
Thermal Cycler Needed, Not included
Gel Electrophoresis Chambers Needed, Not included
Power Supplies Needed, Not included
Gel Trays Needed, Not included
Gel Combs Needed, Not included
Metric Ruler Needed, Not included
Microwave, Heatable Stir Plate, or Boiling Water Bath Needed, Not included
UV Transilluminator, Fluorescent White Light Box, or Blue Light Box Needed, Not included
UV Safety Goggles or Blue Light Safety Goggles or Screen Needed, Not included
Camera Needed, Not included
Household Bleach Solution (10%) or Autoclave Needed, Not included
Gloves Needed, Not included
Semliog Graph Paper Needed, Not included
Lab Coats or Aprons Needed, Not included
Vortex (optional) Needed, Not included
Stereomicroscope or Hand Lens (optional) Needed, Not included

Correlations to State Standards

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    2700 York Road, Burlington, NC 27215-3398 • 800.334.5551