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  • Genotype-Phenotype Connection Extraction and Amplification Kit (with prepaid coupon)

Genotype-Phenotype Connection Extraction and Amplification Kit (with prepaid coupon)

Item # 211217 Exclusive

Introduce your students to a new model organism while building their inquiry skills and advanced molecular techniques. Students use the model microbe Halobacterium sp. NRC-1 to perform DNA extractions, PCR, and gel electrophoresis. Kit contains teacher’s manual and materials for 25 DNA extractions and PCR reactions. Note: Kit also includes a coupon to request shipment of perishable materials later at your convenience (and at no extra charge).

Price

$199.95

In stock and available to ship.
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Overview

Looking for a great way to introduce your students to a new model organism while building their inquiry skills and advanced molecular techniques? In this activity, students use the model microbe Halobacterium sp. NRC-1 to perform deoxyribonucleic acid (DNA) extractions, polymerase chain reactions (PCR), and gel electrophoresis. The activity is designed in inquiry format to allow students to observe and experience the Archaeon and extreme halophile (salt-loving) microorganism, Halobacterium sp. NRC-1, along with a stable mutant derivative, Halobacterium sp. KBT-1. Students identify the phenotypic differences between these colonies and use PCR to analyze DNA from either a mutant (KBT-1), a wild type (NRC-1), or a sectored colony, further cementing the genotype-phenotype connection. Kit contains teacher’s manual with reproducible student guide along with materials for 25 DNA extractions and PCR reactions.

  • This item is only available from Carolina Biological Supply Company.
  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses.

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What's Included

Components Qty Included?
Colonies of Halobacterium sp. NRC-1 (wild type) Included
Colonies of Halobacterium sp. KBT-1 (mutant) Included
PCR Tubes Containing Ready-To-Go-Beads (0.2-mL capacity) 25 Included
Primer/Loading Dye Mix Included
DNA Marker Included
Teacher's Manual with Reproducible Student Guide 1 Included
Pipettes Needed, Not included
Resealable Plastic Bags or Containers Needed, Not included
Fine-Tip Permanent Markers Needed, Not included
Microscope or Hand Lens (optional) Needed, Not included
Microcentrifuge Needed, Not included
Ice Needed, Not included
Vortex (optional) Needed, Not included
Thermal Cycler Needed, Not included
Power Supplies Needed, Not included
Gel Electrophoresis Chambers Needed, Not included
Gel Trays Needed, Not included
Gel Combs Needed, Not included
Microwave or Heatable Stir Plate and Stir Bath or Boiling Waterbath Needed, Not included
UV Transilluminator and Fluorescent White Light Box or Blue light Box Needed, Not included
UV Safety Goggles or Blue Light Safety Goggles or Screen Needed, Not included
Camera to Capture Gel Images Needed, Not included
Bleach Solution (10%) or Autoclave Needed, Not included
Gloves Needed, Not included
Lab Coats or Aprons Needed, Not included

Resources

Teacher Manuals & Student Materials

 

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