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Looking for a great way to introduce your students to a new model organism while building their inquiry skills and advanced molecular techniques? Students use the model microbe Halobacterium sp. NRC-1 to perform DNA extractions, PCR, and gel electrophoresis. Kit contains teacher’s manual with student guide and materials for 25 DNA extractions and PCR reactions, plus materials for electrophoresis using GelGreen™.
Looking for a great way to introduce your students to a new model organism while building their inquiry skills and advanced molecular techniques? In this activity, students use the model microbe Halobacterium sp. NRC-1 to perform deoxyribonucleic acid (DNA) extractions, polymerase chain reactions (PCR), and gel electrophoresis. The activity is designed in inquiry format to allow students to observe and experience the Archaeon and extreme halophile (salt-loving) microorganism, Halobacterium sp. NRC-1, along with a stable mutant derivative, Halobacterium sp. KBT-1. Students identify the phenotypic differences between these colonies and use PCR to analyze DNA from either a mutant (KBT-1), a wild type (NRC-1), or a sectored colony, further cementing the genotype-phenotype connection.
|Halobacterium sp. KBT-1 (mutant)||2||Included|
|Halobacterium sp. NRC-1 (wild type)||3||Included|
|Primer-Loading Dye Mix||1||Included|
|1-kb Ladder/DNA Marker, 30 μL||1||Included|
|GelGreen, 100 μL||1||Included|
|5 M Sodium Chloride, 8 mL||1||Included|
|Sterile Water, 20 mL||2||Included|
|PCR Tubes Containing Ready-To-Go-Beads™ (0.2 mL capacity)||25||Included|
|Agarose, 2 g||1||Included|
|TBE Buffer, 150 mL||1||Included|
|Teacher's Manual and Reproducible Student Guide||1||Included|
|Pipets||Needed, Not included|
|Resealable Plastic Bags or Containers||Needed, Not included|
|Permanent Fine-tip Markers||Needed, Not included|
|Microcentrifuge (with adapters for 0.2 mL tubes)||Needed, Not included|
|Ice||Needed, Not included|
|Thermal Cycler||Needed, Not included|
|Gel Electrophoresis Chambers||Needed, Not included|
|Power Supplies||Needed, Not included|
|Gel Trays||Needed, Not included|
|Gel Combs||Needed, Not included|
|Metric Ruler||Needed, Not included|
|Microwave, Heatable Stir Plate, or Boiling Water Bath||Needed, Not included|
|UV Transilluminator, Fluorescent White Light Box, or Blue Light Box||Needed, Not included|
|UV Safety Goggles or Blue Light Safety Goggles or Screen||Needed, Not included|
|Camera||Needed, Not included|
|Household Bleach Solution (10%) or Autoclave||Needed, Not included|
|Gloves||Needed, Not included|
|Semliog Graph Paper||Needed, Not included|
|Lab Coats or Aprons||Needed, Not included|
|Vortex (optional)||Needed, Not included|
|Stereomicroscope or Hand Lens (optional)||Needed, Not included|
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