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Explore a modern application of genetic engineering through this exciting laboratory activity, which investigates whether the soy or corn ingredients in various processed foods contain a genetic modification. Students isolate DNA from wild-type and genetically modified (GM) plant material (provided controls), and from food products of their choice. Kit includes materials for 25 PCR reactions. Comes with perishable materials.
Advanced - For experienced high school and college classes; requires some basic technical skill.
Explore a modern application of genetic engineering through this exciting laboratory activity. Many commercial crops are genetically modified (GM) to include a transferred gene (transgene) for herbicide resistance. This activity investigates whether the soy or corn ingredients in various processed foods contain a genetic modification. Students isolate DNA from wild-type and GM plant material (provided controls), and from food products of their choice. They use the extracted DNA as a template in 2 separate PCR reactions run under the same conditions. One reaction serves as a positive control to amplify a portion of the tubulin gene found in all plants, and the other assays for genetic modification. Each group works with a plant control and a food product. Kit includes materials for 25 PCR reactions and perishable materials. Note: Kit and perishable components ship separately and may arrive on different days. Order kit to arrive 1 month before performing lab.
|Wild-Type Soybean Seeds||16||Included|
|Roundup Ready® Soybean Seeds||16||Included|
|pBR322/BstN1 Markers, 130 μL||1||Included|
|Tris/EDTA (TE) Buffer with RNase A, 3 mL||1||Included|
|35S Primer/Loading Dye Mix, 500 μL||1||Included|
|Tubulin Primer/Loading Dye Mix, 500 μL||1||Included|
|Edwards' Buffer, 20 mL||1||Included|
|Isopropanol, 100%, 10 mL||1||Included|
|PCR Tubes Containing Ready-To-Go-Beads™ (0.2 mL capacity)||25||Included|
|Mineral Oil, 5 mL||1||Included|
|Agarose, 8 g||1||Included|
|TBE, 150 mL||1||Included|
|CarolinaBLU™ Final Stain, 250 mL||1||Included|
|CarolinaBLU™ Gel and Buffer Stain, 7 mL||1||Included|
|Teacher's Manual and Reproducible Student Guide||1||Included|
|Planting Pot or Flat||Needed, Not included|
|Potting Soil||Needed, Not included|
|Soy or Corn Food Products||Needed, Not included|
|Micropipets and Tips (1 µL to 1000 µL)||Needed, Not included|
|Microcentrifuge Tube Racks||Needed, Not included|
|Microcentrifuge (for 1.5 mL tubes)||Needed, Not included|
|Thermal Cycler||Needed, Not included|
|Water Bath or Heating Block||Needed, Not included|
|Electrophoresis Chambers||Needed, Not included|
|Electrophoresis Power Supplies||Needed, Not included|
|White Light Box (for CarolinaBLU™ stain)||Needed, Not included|
|Permanent Markers||Needed, Not included|
|Container of Crushed Ice||Needed, Not included|
|Camera (optional)||Needed, Not included|
|Vortexer (optional)||Needed, Not included|
|Roundup® Herbicide (optional)||Needed, Not included|
Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.
Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.
At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.
The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.