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Using mitochondrial DNA isolated from their cheek cells or hair, students perform PCR and a restriction enzyme digest to determine their haplotypes with respect to a locus in the mitochondrial genome. Concepts explored in this kit include haplotyping, evolution, and the application of PCR technology.
Advanced - For experienced high school and college classes; requires some technical skill.
Using mitochondrial DNA isolated from their cheek cells or hair, students perform PCR and a restriction enzyme digest to determine their haplotypes with respect to a locus in the mitochondrial genome.
Concepts explored in this kit include haplotyping, evolution, and the application of PCR technology. Kit includes materials for 25 PCR reactions and restriction enzyme digests. Kit also includes a prepaid coupon to request perishable materials later at your convenience. Contact us or return the coupon to request delivery of perishables.
|Testriction Enzyme with Buffer and BSA||Included|
|Ready-to-Go Beads™ (0.2- or 0.5-mL tubes)||Included|
|Sterile Deionized Water||Included|
|Electrophoresis Apparatus||Needed, Not included|
|Thermal Cycler Micropipettors||Needed, Not included|
|1.5 mL Microcentrifuge Tubes||Needed, Not included|
|15 mL Conical Tubes||Needed, Not included|
|Saline Solution||Needed, Not included|
|Paper Cups||Needed, Not included|
Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.
Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.
At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.
The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.