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PTC Extraction, Amplification, and Electrophoresis Kit with CarolinaBLU™ and 0.2-mL Tubes (with Prepaid Coupon)

Item # 211381

This kit explores the molecular basis of the inherited ability to taste the bitter chemical phenylthiocarbamide (PTC). Students determine their ability to taste PTC using taste paper. They then use safe saline mouthwash and Chelex® extraction to obtain a sample of their own DNA and amplify a 221-nucleotide region of the PTC taste receptor gene.

Price

$230.00

In stock and available to ship.

Advanced - For experienced high school and college classes; requires some basic technical skill.

This kit explores the molecular basis of the inherited ability to taste the bitter chemical phenylthiocarbamide (PTC), which was first described in the 1930s by Cold Spring Harbor Laboratory scientist Albert Blakeslee. Students determine their ability to taste PTC using taste paper. They then use safe saline mouthwash and Chelex® extraction to obtain a sample of their own DNA and amplify a 221-nucleotide region of the PTC taste receptor gene. The 2 alleles differ by a single nucleotide, so restriction digestion of the amplified product followed by gel electrophoresis effectively differentiates the 2 alleles. One allele strongly correlates to the ability to taste PTC. After scoring their SNP genotypes, students determine how well the SNP genotypes actually correlate to tasting.

Kit includes materials for 25 PCR reactions and restriction digests, plus a PTC CD-ROM. Kit also includes a prepaid coupon to request perishable materials later at your convenience. Contact us or return the coupon to request delivery of perishables.

   This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. For substitution suggestions, please call us at 800.334.5551 (8am to 8pm, ET, Monday-Friday).

  • This item is sold and shipped to schools and businesses only. Not available to individuals or residential addresses. Visit our Login & Registration page to learn how to create a Login and link it to an approved school or business account.
 

This item includes a prepaid coupon for living or perishable materials (all items). Redeem the coupon for delivery of these materials at your convenience at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Components Qty Included?
pBR322/BstNI Markers, 0.075 μg/μL, 200 μL 1 Included
HaeIII Restriction Enzyme, 40 μL 1 Included
PTC Primer/Loading Dye Mix, 700 μL 1 Included
Chelex® Resin, 1.5 g 1 Included
Ready-to-Go&trade PCR Beads (0.2 mL tubes) 25 Included
Mineral Oil, 5 mL 1 Included
PTC Taste Strips 100 Included
Control Taste Strips 100 Included
PTC Tasting CD-ROM 1 Included
Agarose, 10 g 1 Included
TBE Buffer, 200 mL 1 Included
CarolinaBLU™ Final Stain, 375 mL 1 Included
CarolinaBLU™ Gel and Buffer Stain, 7 mL 1 Included
Latex Gloves 12 Included
Staining Trays 9 Included
Teacher's Manual and Reproducible Student Guide 1 Included
Saline Solution, 0.9% Needed, Not included
Micropipets and Tips, 1 μL to 1000 μL Needed, Not included
Microcentrifuge Tubes, 1.5 mL Needed, Not included
Microcentrifuge Tube Racks Needed, Not included
Microcentrifuge for 1.5 mL Tubes Needed, Not included
PCR Tubes, 0.2 or 0.5 mL Needed, Not included
Tube Adapters for Microcentrifuge, 0.2 or 0.5 mL Needed, Not included
Programmable Thermal Cycler Needed, Not included
Electrophoresis Chambers Needed, Not included
Electrophoresis Power Supplies Needed, Not included
Gel-staining Trays Needed, Not included
White Light Box Needed, Not included
Paper Cups Needed, Not included
Permanent Markers Needed, Not included
Contrainer with Cracked or Crushed Ice Needed, Not included
Boiling Water Bath (optional) Needed, Not included
Camera (optional) Needed, Not included

DNA Learning Center

Cutting Edge Science in your Classroom

Carolina Biological and the Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) have partnered together since 1986 to make molecular genetics and biotechnology more accessible to students globally. Focused on creating innovative classroom activities that give students the opportunity to go hands-on with DNA and explore the core principles of genetics. Together we have constantly worked to increase the accessibility of many key scientific breakthroughs for classrooms globally, including such techniques as PCR, DNA Sequencing, and RNAi.

Bringing a Carolina and DNALC kit into your classroom introduces your students to the experience of scientific research on its highest level, in some cases Nobel Prize winning research, with ease and simplicity. Each kit features in-depth background information, classroom tested protocols, pre-packaged materials, and the knowledgeable technical support of the Carolina Biological team. Our wide selection of kits in this series offers educators' unmatched opportunities to bring biotechnology or genetics into their classroom, with a best in class product.

At Carolina, we believe that innovative research doesn't just belong in a lab—it belongs in your classroom.


About the DNA Learning Center

The DNA Learning Center (DNALC) is the world's first science center devoted entirely to genetics education and is an operating unit of Cold Spring Harbor Laboratory, an important center for molecular genetics research. The mission of the DNA Learning Center is to prepare students and families to thrive in the gene age. We envision a day when all elementary students are exposed to principles of genetics and disease risk; when all high school students have the opportunity to do hands-on experiments with DNA; and when all families have access to genetic information they need to make informed health care choices.

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