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Using an Alu Insertion Polymorphism to Study Human Populations

6 Items
$190.00 - $230.00 View Details

  • Simple, robust protocol is ideal for classroom use
  • Incorporates hardy-Weinberg equilibrium and chi-square analysis
  • Helps demonstrate real-world applications of PCR
  • Discover how Alu insertions are used to track world population migrations
  • CD-ROM contains animations and video clips for classroom instruction

Students use safe saline mouthwash or hair and Chelex extraction to obtain a sample of their own DNA.

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Advanced - For experienced high school and college classes; requires some technical skill.

  • Simple, robust protocol is ideal for classroom use
  • Incorporates hardy-Weinberg equilibrium and chi-square analysis
  • Helps demonstrate real-world applications of PCR
  • Discover how Alu insertions are used to track world population migrations
  • CD-ROM contains animations and video clips for classroom instruction

Students use safe saline mouthwash or hair and Chelex extraction to obtain a sample of their own DNA. Then they use polymerase chain reaction (PCR) to amplify a 300-nucleotide Alu insertion into an intron of H-cadherin gene. Gel electrophoresis seperates the 2 alleles, and each student determines their own genotype. The experiment provides an introduction to human population genetics, as participants learn to score genotypes and calculate genotype and allele frequencies.

Each kit contains instructions, a CD-ROM, and materials for 25 students and is available in 2 versions. The first version, for DNA-extraction and amplification only, includes Chelex, proteinase K, Ready-to-go Beads™ (in 0.2 and 0.5-mL reaction tubes), primer/loading dye mix, mineral oil, and DNA markers. The second version includes all of the above, plus agarose, electrophoresis buffer, staining trays, gloves, and either ethidium bromide or CarolinaBLU™ stain.

Needed But Not Included: Thermal Cycler, Electrophoresis Equipment, Micropipettors, 1.5-mL Microcentrifuge Tubes, 15-mL Conical Tubes, Saline Sloution, Paper Cups.Note: Kits contain perishable materials and are shipped on gel cold packs. Sold only to schools and businesses.

 
 
 
 
 
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