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Using an Alu Insertion Polymorphism to Study Human Populations

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$32.50 - $226.00 View Details

A truly teacher- and student-friendly approach to conducting human genetics studies in your classroom. This high-quality kit gives your students the opportunity to determine their own genotype by looking at a 300-nucleotide Alu insertion (PV92). The experiment is set up to ensure students succeed in making real-world connections between population genetics, evolution, DNA sequencing, and much more. Each kit comes with enough materials for 25 students.

 
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Advanced—For experienced high school and college classes; requires some technical skill.

  • Simple, robust protocol is ideal for classroom use
  • Incorporates Hardy-Weinberg equilibrium and chi-square analysis
  • Helps demonstrate real-world applications of PCR
  • Discover how Alu insertions are used to track world population migrations
  • CD-ROM contains animations and video clips for classroom instruction

Students use safe saline mouthwash or hair and Chelex® extraction to obtain a sample of their own DNA. Then they use polymerase chain reaction (PCR) to amplify a 300-nucleotide Alu insertion into an intron of the H-cadherin gene. Gel electrophoresis separates the 2 alleles, and each student determines his/her own genotype. The experiment provides an introduction to human population genetics as participants learn to score genotypes and calculate genotype and allele frequencies. Each kit contains instructions, a CD-ROM, and materials for 25 students.

Note: Order the kit with the perishable materials included or with a prepaid coupon to request perishables later at your convenience. Contact us or return the coupon to request delivery of perishable materials. Sold only to schools and businesses.

 
 
 
 
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