Each unialgal culture contains enough material for a class of 30 students. For your convenience, we list culture media, optimum growth temperatures, and either Low or High light levels (Low light is 50 to 100 ft candles of fluorescent light or fluorescent light from the ceiling; High light level is 200 to 400 ft candles of fluorescent light 18 to 24" from the culture). Each order of algae includes a FREE Carolina™ Culturing Algae manual.