Sordaria Genetics Class BioKit - Fungi Kits - Fungi - Living Organisms

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Funnel, Polypropylene, 6 inch

Owl Pellet Regional Variation Inquiry Kit

Sordaria Genetics Class BioKit

Note: This kit is available with perishable materials included (all items) or with a prepaid coupon you can use to request delivery of perishables at your convenience. Redeem your coupon 24/7 at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112.

Kit Includes:

Sordaria fimicola Plate Culture (wild type)
Sordaria fimicola Plate Culture (mutant tan)
4 Bottles Sordaria Crossing Agar
Metal Scalpel
Coverslips
Microscope Slides
20 Sterile Petri Dishes
Sordaria Transparency
Toothpicks
Autoclave Disposal Bag
Teacher Instructions and Student Guides

Needed but not included: Microscope

Carolina Teacher Tips

QUESTION: How do I know when my Sordaria plate is ready to use?

ANSWER: Use a wooden toothpick to scrape a few perithecia from the plate periphery, where the 2 strains have grown together. Place the perithecia on a microscope slide, add a drop of water, and gently press down on them with a coverslip. The perithecia should break open with gentle pressure and you should see asci with spores (they resemble bubblegum balls in a wrapper). You should also see a color difference between the 2 spore types in their crossover arrangement.

Here are some signs the Sordaria asci are not ready to be used:

You use a lot of pressure to break open the perithecia (the black spheres).
The spores are greenish in color.
You only see darl, sac-like perithecia and no asci with spores.

If the culture is not ready, continue to incubate the plate at 25° C (77° F) and repeat the testing process until the asci mature and rupture easily on the slide. If the culture is still not ready on Friday, the plate can be refrigerated over the weekend. Allow the plate and culture to come to room temperature before rechecking on Monday.

Intermediate - Easy to perform; requires some basic training in microbiology.

For a class of 30. Students cross the wild-type Sordaria strain with the mutant tan strain. Hybrid asci are produced containing 4 dark- and 4 light-colored ascospores. Students then use tetrad analysis to calculate the gene to centromere distance in map units for the mutant tan gene. The exercise requires about 10 to 14 days from setup of cross plates to completion.

Keep fungi cultures at room temperature; do not incubate or refrigerate.

** Kit and perishable components will ship separately and may arrive on different days.

Item #	Product	Qty	Price

155847	Sordaria Genetics Class BioKit (with Prepaid Coupon)		$106.55




155847P	Sordaria Genetics Class BioKit (with Perishables)		$106.55

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