My Account (Log In)
|
Order Tracking
|
Order by Item #
|
Shopping Lists
|
Shopping Cart
800.334.5551
|
Contact Us
|
Service & Help
|
Online Services
|
International
Search resources,
activities, msds >>
Home
>
Sordaria Genetics Class BioKit
Click image for a larger view.
Sordaria Genetics Class BioKit
The Sordaria Genetics BioKit® includes:
Sordaria fimicola
Plate Culture (wild type)
Sordaria fimicola
Plate Culture (mutant type)
4 Bottles
Sordaria
Crossing Agar
Metal Scalpel
Coverslips
Microscope Slides
20 Sterile Petri Dishes
Instructional Transparency
Toothpicks
Autoclave Disposal Bag
Carolina Teacher Tips
QUESTION:
How do I know when my
Sordaria
plate is ready to use?
ANSWER:
Use a wooden toothpick to scrape a few perithecia from the plate periphery, where the 2 strains have grown together. Place the perithecia on a microscope slide, add a drop of water, and gently press down on them with a coverslip. The perithecia should break open with gentle pressure and you should see asci with spores (they resemble bubblegum balls in a wrapper). You should also see a color difference between the 2 spore types in their crossover arrangement.
Here are some signs the
Sordaria
asci
are not ready to be used:
You use a lot of pressure to break open the perithecia (the black spheres).
The spores are greenish in color.
You only see darl, sac-like perithecia and no asci with spores.
If the culture is not ready, continue to incubate the plate at 25° C (77° F) and repeat the testing process until the asci mature and rupture easily on the slide. If the culture is still not ready on Friday, the plate can be refrigerated over the weekend. Allow the plate and culture to come to room temperature before rechecking on Monday.
Intermediate
- Easy to perform; requires some basic training in microbiology.
A cross of the wild-type
Sordaria
strain with the mutant tan strain is made. Hybrid asci are produced containing 4 dark- and 4 light-colored ascospores. Tetrad analysis is used to calculate the gene to centromere distance in map units for the mutant tan gene.
The exercise requires about 10 to 14 days from setup of cross plates to completion. Requires the use of a compound microscope. This kit is designed for a class of 30 students. With instructions.
Kit contains coupon for prepaid delivery of perishable materials. Please return coupon at least 2 weeks prior to the requested delivery date to ensure prompt delivery.
Item #
Product
Qty
Price
155847
Sordaria Genetics Class BioKit
$99.95
Buy Fast. Feel Secure. 100% Satisfaction Guaranteed.
Payment Options
Payment Options
Purchasing from Carolina Biological Supply is safe, quick and easy. We accept all forms of payment:
Visa
,
Mastercard
,
American Express
,
Discover
, and
Purchase Orders
.
Your privacy and security are important to us. We fully utilize VeriSign SSL technology to keep your personal information safe.
Purchase Orders
Purchase Orders
Carolina also offers the ability to use a Purchase Order as your online payment method—and it's fast and easy to set up.
If this is your first visit and you qualify for purchase order payments,
please call 800.334.5551 (7am-8pm, ET, Mon-Fri) so we can create your personal Carolina online account.
It only takes a few minutes, and once your login is ready, you can use a purchase order during checkout.
Tax Exemption
Tax Exemption
Sales tax exemption is available for qualified customers—just have your tax exemption certificate ready.
If this is your first visit and you qualify for tax exemption,
please call 800.334.5551 (7am-8pm, ET, Mon-Fri) so we can create your personal Carolina online account.
It only takes a few minutes, and once your login is ready, we'll remove tax from your online orders.
Shipping Options
Shipping Options
We offer three convenient shipping methods to meet your timeline:
Standard
,
2nd Day
, and
Next Day
. We get it to you when you need it!
Note that living and perishable materials automatically ship via 2nd Day Air and will arrive on the date(s) you select in your Shopping Cart.
Sordaria Demonstration Cross Plate, Living
$21.95
Chromosome Mapping Drosophila BioKit
$121.00
Drosophila, Living, Two Point Linkage
$20.75
/product/living+organisms/fungi/fungi+kits/sordaria+genetics+class+biokit.do
Contact Us
|
The Carolina Pledge
|
About Us
|
Conferences
|
Find a Friend's List
Privacy & Security
|
Terms of Use
|
Site Map
2700 York Road, Burlington, NC 27215-3398 800.334.5551
©2010 Carolina Biological Supply Company