Note: Each kit contains a coupon for prepaid delivery of perishable materials. Contact us or return the coupon for prepaid delivery of perishable material. If you need perishables to arrive with kit, please specify whe nordering. Sold only to schools and businesses. Kits Include: 4-Station Kit 8-Station Kit Teacher Demo Kit Perishables Refill E. coli 1 1 1 1 200 µL Plasmid Solution 1 1 1 1 4 mL Ampicillin 1 1 1 400 mL Sterile LB Agar 1 2 100 x 15-mm Petri Dishes 20 40 Prepoured AMP Plates 2 2 (* with demo refill only) Prepoured LB Plates 3 3 (* with demo refill only) 3 mL LB Broth 4 8 1 3 mL Transformation Buffer 4 8 1 Pack of Glass Beads 1 1 1 Transfer Bulb Pipets 24 48 7 Transformation Tubes 8 16 2 Transfer Loops, Sterile 15 28 4 Wire Inoculating Loop 1 1 1 1 Instruction Booklet w/Student Masters 1 1 1 Needed But Not Included: Containers of ice, 37° Incubator (optional), Method for maintining 42° C for 90 seconds, Long-Wave UV Light (optional)

Intermediate - Easy to perform; requires some background knowledge.

• Green color is clearly visible in normal classroom light
• No UV lighting needed
• Pre-aliquoted and prepared reagents minimize teacher prep time

Open your students' eyes to the genetic basis of inheritance with this simple lab. Developed in cooperation with the Dolan DNA Learning Center, these kits clearly demonstrate the induced uptake of a jellyfish gene by E. coli. Students observe the phenotypic effect of adding the gene for GFP (green fluorescent protein). GFP is expressed on plain LB - no color development substrate or induction reagent is needed. Your students can easily view the "green" effects in normal classroom lighting. Colonies will fluoresce under UV light. The plasmid containing the GFP gene also contains an ampicillin resistance gene, so ampicillin can be used to select for transformants. Lab procedure is easy to follow.