This classic lab was developed in cooperation with the Dolan DNA Learning Center.
Students perform restriction digests on bacteriophage lambda DNA, conduct electrophoretic analysis of the reactions, and stain the resulting gel. They then observe and analyze the stained patterns of fragments separated by size during gel electrophoresis.
Materials are sufficient for 6 teams of students or 6 separate instructor demonstrations.
Refills include lambda DNA, 2x restriction buffer, and 15µL of restriction enzymes (
BamH1,
EcoRI, and
HindIII).
** Kit and perishable components will ship separately and may arrive on different days.