Materials are sufficient for 25 reactions, or 6 to 8 time-course experiments. Kits contain perishable materials and are shipped on gel cold packs. Sold only to schools and businesses. Amplification Kits include: Lambda DNA Lambda PCR Primers/Loading Dye Mixture 25 Ready-to-Go Beads™ and DNA Markers Instructions Amplification and Electrophoresis Kits also contain: Electrophoresis Buffer and Agarose, Staining Solution and Trays, Disposable Gloves Needed but not Included: 2 Water Baths (55° and 100° C) or DNA Thermal Cycler, Gel Electrophoresis Chamber and Power Supply, Micropipettors

Polymerase Chain Reaction - Human DNA PCR Kits Each kit uses a 30-minute method that requires no specialized equipment to isolate template DNA from hair sheaths or cheek (buccal) cells Simply add student DNA template and primer mix to the ready-to-go Beads™ which incorporate the needed components and are stable at room temperature. Saves time and ensures accuracy To save additional time, loading dye is incorporated into the primer mix - amplified reactions can be taken from the thermal cycler and immediately loaded for gel electrophoresis. (Human haplotyping kit requires that loading dye be added after the final step). PCR products can be readily analyzed on agarose and can be stained with either ethidium bromide or CarolinaBLU™ Why Carolina™ PCR Kits? Highest Quality - Carolina's staff works with the experts at the Dolan DNA Learning Center (DNALC) of Cold Spring Harbor and other collaborators to develop the very best kits available. Teacher and Student Friendly - We've taken measures to save you prep time and make the labs convenient Carolina™ Service and Guarantee - The best assurance you can have. We offer free online and telephone support. If you have a question, we'll answer it promptly. If you have a problem with any of our materials, we'll do whatever it takes to fix it - guaranteed!

Intermediate - Easy to perform; requires some background knowledge.

Good for beginning PCR study. Students amplify a 1,106-bp sequence from the bacteriophage lambda genome using a sample of dilute lambda DNA mixed with PCR Ready-to-Go Beads™ and lambda PCR primers. The mixture can be amplified using 2 water baths (55° C and 100° C) or by using a DNA thermal cycler.

Students load the PCR samples onto a 1% agarose gel, electrophorese, and stain. They then verify the size of the amplified product by comparing it to DNA size markers. This kit can be used to do time-course studies in which the number of amplification cycles is varied.

Students can then observe a qualitative difference in the intensity of the bands on the gel in response to the number of amplification cycles performed.