Use this kit to teach: Population Genetics Hardy-Weinberg equilibrium Forensics techniques The repeated structure of the VNTR polymorphism makes it more difficult to amplify - so this experiment greatly benefits from a "hot start", in which one of the reagents is added during the first denaturing cycle. Each Kit Contains instructions and materials for 25 students and is available in 2 versions. The First Version, for DNA extraction and amplification only, includes Chelex, proteinase K, Ready-to-Go Beads (in 0.2- or 0.5-mL reaction tubes), mineral oil, primer/loading dye mix, and DNA markers. The Second Version includes all the above plus agarose, electrophoresis buffer, staining trays, gloves, and either ethidium bromide or CarolinaBLU™ stain. Needed But Not Included:1.5-mL Microcentrifuge Tubes, 15-mL Conical Tubes, Saline Solution, Paper Cups, Thermal Cycler, Electrophoresis Equipment, Micropipets.

Polymerase Chain Reaction - Human DNA PCR Kits Each kit uses a 30-minute method that requires no specialized equipment to isolate template DNA from hair sheaths or cheek (buccal) cells Simply add student DNA template and primer mix to the ready-to-go Beads™ which incorporate the needed components and are stable at room temperature. Saves time and ensures accuracy To save additional time, loading dye is incorporated into the primer mix - amplified reactions can be taken from the thermal cycler and immediately loaded for gel electrophoresis. (Human haplotyping kit requires that loading dye be added after the final step). PCR products can be readily analyzed on agarose and can be stained with either ethidium bromide or CarolinaBLU™ Why Carolina™ PCR Kits? Highest Quality - Carolina's staff works with the experts at the Dolan DNA Learning Center (DNALC) of Cold Spring Harbor and other collaborators to develop the very best kits available. Teacher and Student Friendly - We've taken measures to save you prep time and make the labs convenient Carolina™ Service and Guarantee - The best assurance you can have. We offer free online and telephone support. If you have a question, we'll answer it promptly. If you have a problem with any of our materials, we'll do whatever it takes to fix it - guaranteed!

Biotech Kit Instruction Manuals Whether you're considering this product for your classroom or just need another copy of the manual, you'll find many of our most popular Biotechnology Kit manuals here. The link will open a new browser window, so please disable popup blockers.

Advanced - For experienced high school and college classes; requires some technical skill.

• Provides an introduction to the use of PCR in forensics
• Helps demonstrate real-world applications of PCR

This lab illustrates the use of DNA typing to identify individuals in court cases and disasters. It assays for variable numbers of tandem repeats (VNTR) polymorphisms, which are caused by short, repeated copies of a 16-nucleotide sequence at the pMCT118 locus. Differences in the number of repeated units produce longer and shorter alleles, which can be resolved by gel electrophoresis.

Because the VNTR locus has more than 29 different alleles, a panel of student types shows a variety of different genotypes.