DNALC—Developed in collaboration with the Dolan DNA Learning Center, Cold Spring Harbor Laboratory. Amplification Kits include: Chelex Proteinase K Ready-to-Go Beads™ (0.2- or 0.5-mL tubes) Primer Mix Loading Dye DNA Marker Restriction Enzyme with Buffer and BSA Microcentrifuge Tubes Sterile Deionized Water Mineral Oil Amplification and Electrophoresis Kits also contain: Agarose and Electrophoresis Buffer Staining Trays and Gloves CarolinaBLU™ Stain Needed But Not Included:1.5-mL Microcentrifuge Tubes, 15-mL Conical Tubes, Saline Solution, Paper Cups.

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Using mitochondrial DNA isolated from their cheek cells or hair, students perform PCR and a restriction enzyme digest to determine their haplotypes with respect to a locus in the mitochondrial genome. Concepts explored in this kit include haplotyping, evolution, and the application of PCR technology. Includes materials for 25 PCR reactions and restriction enzyme digests.
Note: Kit contains coupon for prepaid delivery of perishables, unless you request delivery of the perishables with kit. Students use PCR and restriction enzyme analysis to determine their own mitochondrial haplotype.