Each kit contains instructions, a CD-ROM, and materials for 25 students and is available in 2 versions. The first version, for DNA-extraction and amplification only, includes Chelex, proteinase K, Ready-to-go Beads™ (in 0.2 and 0.5-mL reaction tubes), primer/loading dye mix, mineral oil, and DNA markers. The second version includes all of the above, plus agarose, electrophoresis buffer, staining trays, gloves, and either ethidium bromide or CarolinaBLU™ stain. Needed But Not Included: Thermal Cycler, Electrophoresis Equipment, Micropipettors, 1.5-mL Microcentrifuge Tubes, 15-mL Conical Tubes, Saline Sloution, Paper Cups. Note: Kits contain perishable materials and are shipped on gel cold packs. Sold only to schools and businesses.

Polymerase Chain Reaction - Human DNA PCR Kits Each kit uses a 30-minute method that requires no specialized equipment to isolate template DNA from hair sheaths or cheek (buccal) cells Simply add student DNA template and primer mix to the ready-to-go Beads™ which incorporate the needed components and are stable at room temperature. Saves time and ensures accuracy To save additional time, loading dye is incorporated into the primer mix - amplified reactions can be taken from the thermal cycler and immediately loaded for gel electrophoresis. (Human haplotyping kit requires that loading dye be added after the final step). PCR products can be readily analyzed on agarose and can be stained with either ethidium bromide or CarolinaBLU™ Why Carolina™ PCR Kits? Highest Quality - Carolina's staff works with the experts at the Dolan DNA Learning Center (DNALC) of Cold Spring Harbor and other collaborators to develop the very best kits available. Teacher and Student Friendly - We've taken measures to save you prep time and make the labs convenient Carolina™ Service and Guarantee - The best assurance you can have. We offer free online and telephone support. If you have a question, we'll answer it promptly. If you have a problem with any of our materials, we'll do whatever it takes to fix it - guaranteed!

Advanced - For experienced high school and college classes; requires some technical skill.

• Simple, robust protocol is ideal for classroom use
• Incorporates hardy-Weinberg equilibrium and chi-square analysis
• Helps demonstrate real-world applications of PCR
• Discover how Alu insertions are used to track world population migrations
• CD-ROM contains animations and video clips for classroom instruction

Students use safe saline mouthwash or hair and Chelex extraction to obtain a sample of their own DNA. Then they use polymerase chain reaction (PCR) to amplify a 300-nucleotide Alu insertion into an intron of H-cadherin gene. Gel electrophoresis seperates the 2 alleles, and each student determines their own genotype. The experiment provides an introduction to human population genetics, as participants learn to score genotypes and calculate genotype and allele frequencies.