Note: This kit is available with perishable materials included (all items) or with a prepaid coupon you can use to request delivery of perishables at your convenience. Redeem your coupon 24/7 at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112. Kits Include: 4-Station Kit 8-Station Kit Teacher Demo Kit Perishables Refill E. coli 1 1 1 1 200 µL Plasmid Solution 1 1 1 1 4 mL Ampicillin 1 1 - 1 (not included in Demo Kit Refill) 400 mL Sterile LB Agar 1 2 - - 100 x 15-mm Petri Dishes 20 40 - - Prepoured AMP Plates - - 2 2 (Demo Kit Refill only) Prepoured LB Plates - - 3 3 (Demo Kit Refill only) 3 mL LB Broth 4 8 1 - 3 mL Transformation Buffer 4 8 1 - Pack of Glass Beads 1 1 1 - Transfer Bulb Pipets 24 48 7 - Transformation Tubes 8 16 2 - Transfer Loops, Sterile 15 28 4 - Wire Inoculating Loop 1 1 1 - Instruction Booklet w/Student Masters 1 1 1 - Needed But Not Included: Containers of ice, 37° Incubator (optional), Method for maintining 42° C for 90 seconds, Long-Wave UV Light (optional)

Carolina Leads the Way in Kits for AP® Biology! First complete kits available for all 12 AP® Biology Labs Developed in collaboration with leading experts Flexible to fit your class size Pioneering Innovation and Continuing Support - We were the first to offer kits specifically designed for each of the 12 AP® Biology Labs. Working closely with experts such as Dr. Bill Barstow, one of the original AP® Lab developers, we designed our kits to meet all the objectives of every lab. We have also revised our kit instructions to enhance clarity and provide questions that challenge students to think critically rather than memorize facts. Our continuing support of AP® Biology includes loaning equipment and donating kits for teacher training at AP® Biology Summer Institutes. More teachers have been trained using out kits than those of any other supplier. Technical Support for all AP Biology Laboratories is easily accessible - Call Tim Woody at 800.227.1150, we welcome your suggestions.

This kit is great for teaching:

• The molecular basis of heredity
• The relationship between genes and proteins
• How E. coli is used to engineer proteins useful in medicine and manufacturing

The lab is set up in 3 modules that can be purchased separately and used in conjunction with one another.
Module 1, the Green Gene Colony Transformation Kit, is necessary for creating the GFP-expressing bacteria used in the protein purification exercise in Module 2.
Module 2 contains all the reagents and instructions necessary for purifying GFP from E. coli.
Module 3 contains the acrylamide gels and other electrophoresis reagents necessary for electrophoretic analysis of the purified GFP. The 3rd module is optional but increases the teaching value of the lab.

** Kit and perishable components will ship separately and may arrive on different days.