Amplification Kits include: Wild-Type and GM Plant Material Edward's Buffer Isopropanol Tris-EDTA (TE) Buffer with RNase 2 Primer/Loading Dye Mixes Ready-to-Go Beads™ (0.2- or 0.5-mL tubes) Mineral Oil DNA Marker Pellet Pestles Microcentrifuge Tubes Teacher's Manual with Reproducible Student Guide Amplification and Electrophoresis Kits also contain: Agarose and Electrophoresis Buffer Staining Trays and Gloves Ethidium Bromide or CarolinaBLU™ Stain Needed but not included: Electrophoresis Apparatus, Thermal Cycler, Micropipettors, Food Products, 1.5-mL Microcentrifuge Tubes.

Polymerase Chain Reaction - Human DNA PCR Kits Each kit uses a 30-minute method that requires no specialized equipment to isolate template DNA from hair sheaths or cheek (buccal) cells Simply add student DNA template and primer mix to the ready-to-go Beads™ which incorporate the needed components and are stable at room temperature. Saves time and ensures accuracy To save additional time, loading dye is incorporated into the primer mix - amplified reactions can be taken from the thermal cycler and immediately loaded for gel electrophoresis. (Human haplotyping kit requires that loading dye be added after the final step). PCR products can be readily analyzed on agarose and can be stained with either ethidium bromide or CarolinaBLU™ Why Carolina™ PCR Kits? Highest Quality - Carolina's staff works with the experts at the Dolan DNA Learning Center (DNALC) of Cold Spring Harbor and other collaborators to develop the very best kits available. Teacher and Student Friendly - We've taken measures to save you prep time and make the labs convenient Carolina™ Service and Guarantee - The best assurance you can have. We offer free online and telephone support. If you have a question, we'll answer it promptly. If you have a problem with any of our materials, we'll do whatever it takes to fix it - guaranteed!

Explore a modern application of genetic engineering through this exciting laboratory activity. This activity investigates whether the soy or corn ingredients in various processed foods contain a genetic modification. Students isolate DNA from wild-type and GM plant material (provided controls), and from food products of their choice. They use the extracted DNA as a template in 2 seperate PCR reactions run under the same conditions. One reaction serves as a positive control to amplify a portion of the tubulin gene found in all plants, and the other assays for genetic modification. Each group works with a plant control and a food product.

Each kit includes materials for 6 groups (25 PCR reactions).

Kit contains coupon for prepaid delivery of perishable materials. Contact us or return coupon to request delivery of perishable materials. If you need perishable to arrive with kit, please specify when ordering.

Allow 1 month lead time for growth of control plants for PCR.