Using a Single Nucleotide Polymorphism (SNP) to Predict Bitter Tasting Ability - Polymerase Chain Reaction (PCR) - Biotechnology Kits & Materials - Life Science

Using a Single Nucleotide Polymorphism (SNP) to Predict Bitter Tasting Ability

Using a Single Nucleotide Polymorphism (SNP) to Predict Bitter Tasting Ability

This kit explores the molecular basis of the inherited abilit yto taste the bitter chemical phenylthiocarbamide (PTC), which was first described in the 1930's by Cold Spring Harbor Laboratory scientist Albert Blakeslee.

Students determine their ability to taste PTC using taste paper. They then use safe saline mouthwash and Chelex extraction to obtain a sample of their own DNA and amplify a 221-nucleotide region of the PTC taste receptor gene.

The 2 alleles differ by a single nucleotide, so restriction digestion ofthe amplified product follwed by gel electrophoresis effectively differentiates the 2 alleles. One allele strongly correlates to the ability to taste PTC. After scoring their SNP genotypes, students determine how well the SNP genotypes actually correlate to tasting.

Item#	Product	Qty	Price

211304	PTC CD-ROM	We regret that this item is not currently available.




211376	PTC PCR DNA Extraction and Amplification Kit with 0.5-mL Tubes		$169.75




211377	PTC PCR DNA Extraction and Amplification Kit with 0.2-mL Tubes		$169.75




211378	PTC PCR DNA Extraction, Amplification, and Electrophoresis Kit with Ethidium Bromide and 0.5-mL Tubes		$189.00




211379	PTC PCR DNA Extraction, Amplification, and Electrophoresis Kit with Ethidium Bromide and 0.2-mL Tubes		$189.00




211380	PTC PCR DNA Extraction, Amplification, and Electrophoresis Kit with CarolinaBLU and 0.5-mL Tubes		$179.00




211381	PTC PCR DNA Extraction, Amplification, and Electrophoresis Kit with CarolinaBLU and 0.2-mL Tubes		$179.00


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