Purification of Green Fluorescent Protein Kit - Transformation and Advanced Techniques - Biotechnology Kits & Materials - Life Science

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Purification of Green Fluorescent Protein Kit

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DNA Structure and Function Kit


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Crayfish Structure and Function Kit

Purification of Green Fluorescent Protein Kit

Module 1, is necessary for creating the GFP-expressing bacteria used in the protein purification exercise in Module 2.

Module 2 contains all the reagents and instructions necessary for purifying GFP from E. coli.

Module 3 contains the acrylamide gels and other electrophoresis reagents necessary for electrophoretic analysis of the purified GFP.

Module 1 (8-Station Kit) includes:
E. coli
200 µL Plasmid DNA (0.005 µg/µL)
8 vials Transformation Buffer (3 mL per vial)
8 vials LB Broth (3 mL per vial)
Ready-to-Pour Plates (for 40 plates)
4 mL Ampicillin (10 mg/ml)
1 oz Spreading Beads
28 Sterile Loops
16 Transformation Tubes
48 Sterile Bulb Pipets
Wire Inoculating Loop
Instructions with Student Master Sheets

Module 2 (8-Station Kit):
8 Tubes HIC Resin (300 µL per tube)
8 Tubes Lysis Buffer (600 µL per tube)
8 Tubes Binding Buffer (400 µL per tube)
8 Tubes Wash Buffer (600 µL per tube)
8 Tubes elution buffer (600 µL per tube)
20 mL LB Broth
4 mL Ampicillin (10 mg/mL)
8 Culture Tubes
48 Microcentrifuge Tubes
10 mL Equilibration Buffer
Instructions with Student Master

Module 3 (8-Station Kit):
3 Polyacrylamide Gels
500 mL 5x Tris-Glycine SDS Buffer
30 µL Protein Marker
24 Microcentrifuge Tubes
4 Tubes Sample Loading Buffer (100 µL per tube)
500 mL COOMASSIE® Stain
3 Staining Trays
Instructions with Student Manual

Teach your students how to purify a green fluorescent jellyfish protein produced in E. coli!

This kit is great for teaching:

The molecular basis of heredity
The relationship between genes and proteins
How E. coli is used to engineer proteins useful in medicine and manufacturing
Three techniques – transformation, protein purification, and protein gel electrophoresis, commonly used in biological research and its areas of application

Note:Module 1 contains a coupon for prepaid delivery of perishable items. Please contact us or return coupon 2 weeks prior to requested delivery date to ensure prompt arrival of materials. Sold only to schools and businesses.
Needed, not included:Equipment for Growing Bacterial Suspension Cultures, Pipets, Microcentrifuge.

Item#	Product	Qty	Price

211082	AP Biology Lab 6: Molecular Biology - Green Gene Colony Transformation 8-Station Student Kit		$102.00




211072	Purification of Green Fluorescent Protein 8-Station Student Kit, Module 2		$43.00




211073	Purification of Green Fluorescent Protein 8-Station Student Kit, Module 3		$83.00

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