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Glow-in-the-Dark Transformation Kit
Glow-in-the-Dark Transformation Kit
Bacteria that produce light are very common in the ocean. One luminescent bacterium is
Vibrio fischeri
. The group of genes involved in the production of light (also known as the
lux
genes) has been removed from
V. fischeri
and placed in a plasmid, pVIB. Bacterial transformation is used to transfer the pVIB plasmid into
E. coli
. After
E. coli
takes up the plasmid, it glows in the dark. The transformed colonies are also ampicillin resistant.
Item#
Product
Qty
Price
211086
Glow-in-the-Dark Transformation Teacher Demo Kit
$47.00
211086C
Glow-in-the-Dark Colony Transformation Teacher Demonstration Kit Perishables Refill
$18.00
211087
Glow-in-the-Dark Transformation 4-Station Student Kit
$59.00
211088
Glow-in-the-Dark Transformation 8-Station Student Kit
$99.00
211088C
Glow-in-the-Dark Transformation 4- and 8-Station Kit Perishables Refill
$19.00
Note:
Bacteria transformed with the pVIB plasmid must be grown at 30° C or less for the glowing to be seen. Each kit contains a coupon for prepaid delivery of perishable materials. Contact us or return the coupon at least 2 weeks prior to requested delivery date to ensure prompt arrival of materials. When ordering, specify if you need perishables to arrive with kit.
Teacher Demonstration Kit and Student Kits include:
E. coli
and Plasmid DNA Solution
Transformation Buffer and Luria Broth
Ready-to-Pour Plates (Demonstration Kit contains prepoured plates)
Ampicillin
Spreading Beads and Sterile Tubes
Pipets and Transfer Loops
Teacher's Manual with Student Master Sheets
All Perishable Refills include:
Ampicillin
E. coli
Plasmid DNA Solution
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