Note: This kit is available with perishable materials included (all items) or with a prepaid coupon you can use to request delivery of perishables at your convenience. Redeem your coupon 24/7 at www.carolina.com/livingform, by phone at 800.334.5551, or by fax at 800.222.7112. Teacher Demonstration Kit and 4- and 8-Station Kits include: E. coli pBLU® Plasmid Transformation Buffer and Luria Broth Ready-to-Pour Plates (Demonstration Kit contains prepoured plates) Ampicillin Prepared Ampicillin/X-gal Solution Spreading Beads and Sterile Tubes Pipets and Transfer Loops Teacher’s Manual with Student Masters 4- and 8-Station Perishables Refills include: E. coli Plasmid DNA Solution Ampicillin Prepared Ampicillin/X-gal Solution

Carolina Leads the Way in Kits for AP® Biology! First complete kits available for all 12 AP® Biology Labs Developed in collaboration with leading experts Flexible to fit your class size Pioneering Innovation and Continuing Support - We were the first to offer kits specifically designed for each of the 12 AP® Biology Labs. Working closely with experts such as Dr. Bill Barstow, one of the original AP® Lab developers, we designed our kits to meet all the objectives of every lab. We have also revised our kit instructions to enhance clarity and provide questions that challenge students to think critically rather than memorize facts. Our continuing support of AP® Biology includes loaning equipment and donating kits for teacher training at AP® Biology Summer Institutes. More teachers have been trained using out kits than those of any other supplier. Technical Support for all AP Biology Laboratories is easily accessible - Call Tim Woody at 800.227.1150, we welcome your suggestions.

Students use specially developed pBLU® plasmid in a colony transformation procedure to observe the phenotypic effect of inserting new genes into living bacteria. An ampicillin-sensitive strain of E. coli, incapable of producing β-galactosidase for lactose breakdown, is induced to take up pBLU® plasmid DNA. This plasmid contains genes for both ampicillin resistance and β-galactosidase production. The transformed cells are plated on (1) medium containing ampicillin, and (2) medium containing ampicillin and X-gal. (X-gal is a histochemical substrate that, when cleaved by β-galactosidase, forms a blue product.) Transformants appear as white colonies on the ampicillin medium and as blue colonies on the ampicillin/X-gal medium. The transformed colonies’ phenotype is lac+ and ampr.

** Kit and perishable components will ship separately and may arrive on different days.