Note: Each kit contains a coupon for prepaid delivery of perishable materials. Contact us or return the coupon to request delivery of perishable materials. If you need perishables to arrive with kit, please specify when ordering. Sold only to schools and businesses. Teacher Demonstration Kit and Student Kits include: E. coli and pBLU® Plasmid Transformation Buffer and Luria Broth Ready-to-Pour Plates (Demonstration Kit contains prepoured plates) Ampicillin and Prepared X-gal Solution Spreading Beads and Sterile Tubes Pipets and Transfer Loops Teacher's Manual with Student Master Sheets Perishables Refill includes: Ampicillin, E. coli, Plasmid DNA Solution, Ampicillin/X-gal.

Carolina Leads the Way in Kits for AP® Biology! First complete kits available for all 12 AP® Biology Labs Developed in collaboration with leading experts Flexible to fit your class size Pioneering Innovation and Continuing Support - We were the first to offer kits specifically designed for each of the 12 AP® Biology Labs. Working closely with experts such as Dr. Bill Barstow, one of the original AP® Lab developers, we designed our kits to meet all the objectives of every lab. We have also revised our kit instructions to enhance clarity and provide questions that challenge students to think critically rather than memorize facts. Our continuing support of AP® Biology includes loaning equipment and donating kits for teacher training at AP® Biology Summer Institutes. More teachers have been trained using out kits than those of any other supplier. Technical Support for all AP Biology Laboratories is easily accessible - Call Mary Haugen at 800.227.1150 or Tim Woody at 800.227.1150, we welcome your suggestions.

Intermediate - Easy to perform; requires some background knowledge.

• Great for teaching the molecular basis of inheritance
• Simple, straightforward procedure
• Pre-aliquoted and prepared reagents minimize teacher prep time

Students use pBLU® plasmid in a colony transformation procedure to observe the phenotypic effect of inserting new genes into living bacteria. An ampicillin-sensitive strain of E. coli, incapable of producing ß-galactosidase for lactose breakdown is induced to take up pBLU® plasmid DNA. The transformed cells are plated on (1) madium containing ampicillin, and (2) medium containing ampicillin and X-gal. (X-gal is a histochemical substrate that, when cleaved by β-galactosidase, forms a blue product). Transformants appear as white colonies on the ampicillin madium and as blue colonies on the ampicillin/X-gal medium. The transformed colonies' phenotype is lac+ and amp^r.